FastQCFastQC Report
Mon 10 Jul 2023
SRR6398769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101020049
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1224028112.116684877078212No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT29676412.9376752727569952No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT28412612.8125713936250416No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT26078172.5814845922317855No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT21134052.0920649127778588No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC18378231.819265599445512No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC17691001.7512365292952885No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16204191.604056834302268Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10768431.0659695878785407No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC9509600.9413576902937357No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC9203050.9110122288695386No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA8617990.8530969926573685No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6881000.68115191668537No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6582000.651553831655734No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6201950.6139325867877969No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG5887520.5828070821862301No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA5776860.5718528210177368No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG5008780.49582038907939946No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA4657260.4610233360706447No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA4201780.41593525657466274No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4183350.4141108662499263No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA3963510.3923488494843237No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA3929240.38895645358477304No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA3716780.3679249848710725No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA3083950.30528098437172607No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA2787740.27595908214219933No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG2422770.23983061025836566No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA2301400.22781616350235584No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG2186600.21645208269499058No Hit
TGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2030810.2010303915017899No Hit
GCATGGAATAATAGAATCTGTAGGCACTATCAATAGATCGGAAGAGCACA2026480.20060176371524036No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA2021480.20010681246056414No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1896280.1877132330434724No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1885290.18662533018569413No Hit
GCATGGAATAATAGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACA1831720.18132242244309346No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1802940.1784734830211773No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1729720.1712254168476992No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACC1709220.16919611670352686No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1707540.16902981308195564No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG1705390.16881698404244488No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG1674020.16571165987060646No Hit
GCATGGAATAATAGAATCTGTAGGCACCATTAATAGATCGGAAGAGCACA1670540.16536717379735186No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG1669930.16530678974428134No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1505300.1490100247328132No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1476410.14615019638329416No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1453900.14392192583474198No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA1447980.14333590354920536No Hit
ATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1433820.1419342015959624No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1353870.13401993103369017No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA1347800.13341906021051325No Hit
CATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGA1347490.13338837323272335No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1307850.12946439968565052No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1286070.12730839202028105No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1238560.12260536519834792No Hit
CATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACA1231560.12191243344180124No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1139360.11278553230557234No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1132580.11211437840423141No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA1114790.11035334184009354No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1112810.11015734114324177No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1096690.10856161829816574No Hit
CATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1080750.10698371369825806No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1079220.10683225861432716No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT1078850.1067956322214811No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA1064420.10536720290048564No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1044880.10343293339721109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)