FastQCFastQC Report
Tue 4 Jul 2023
SRR6398770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63590311
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT38060885.985326915605114No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT34691195.455420716530227No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA33634305.289217723750399No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23833183.7479263153784546No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT21278593.346200020943442No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA18974032.9837926095376384Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA16861502.65158319480463No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT10418501.6383785259361288No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC8741521.3746622500399472No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7417971.166525195953201No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA7320471.1511926714747471No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA5882600.9250780358661873No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA4585900.7211633231358154No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG4570710.718774594450403No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3746050.5890913161283328No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3628720.5706403920559533No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3581590.5632288856080606No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3186460.5010920610216861No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2842210.4469564553631449No Hit
TTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2381810.3745554884925787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG2347450.3691521496097102No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA2067650.32515173577308026No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2012270.3164428618693184No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1959620.30816329865095327No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1957760.30787080126090277No Hit
TGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1895950.29815076702486953No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1889390.2971191633266269No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA1687870.2654288009379291No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACC1666610.2620855243183195No Hit
TCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1639250.25778298206467337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1636650.2573741147452479No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1554410.24444132691849862No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1520530.23911347123306256No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1430710.2249886779135268No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1399880.22014045504510898No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1396910.2196734027609961No Hit
TTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1394890.21935574430513477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1365860.21479058342708846No Hit
TGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1268030.19940616425039973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1240150.19502184853286847No Hit
GCATGGAATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1149990.1808435879484848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGAATAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1113800.17515246937540532No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1108440.17430957367074365No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1066230.16767176999653297No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1062040.16701286458561274No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1048010.16480655362732854No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1022930.16086255656148624No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG963400.15150106751325687No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT925480.14553789491609814No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA897550.14114571636550102Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT889260.13984205864317914No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA858100.13494194107652657No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA830080.13053560942641088No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA785410.12351095436535921No Hit
GGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC777880.12232681170563861No Hit
TCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT769480.12100585575057182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC755350.11878381912615588No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG749150.1178088278259875No Hit
TTGATCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT698600.10985950359638909No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG690820.10863604677133912No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA677060.10647219511161063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)