FastQCFastQC Report
Wed 5 Jul 2023
SRR6398771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90339571
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA83958259.293629477164552No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT53498225.921903259868259No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT32825603.6335793536145973No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT30710493.3994505021503807No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23799262.6344225167949933No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC20576332.277665232658676No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT19627972.1726879796672933No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA15869851.7566886608305898No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10979361.215343384794245Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA10974081.2147589233072627No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC10099131.1179076774672752No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA9982511.1049986057604813No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG8739850.9674442664776436No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC8033230.8892260513391191No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7103520.7863132314409595No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6089070.6740202474505884No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC5732980.6346034120529529No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG5369920.5944150432151155No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG5245560.5806492041012681No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA5003950.5539045563986572No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG4839810.535735331309023No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3667310.40594724542138905No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA3381120.374267883118462No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA3356250.3715149366826194No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA3339170.36962429232700256No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3217920.35620270988446473No Hit
TGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3185660.3526317387537738No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG3025530.3349063944525484No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA2611790.28910808088738876No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG2605380.2883985357867152No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC2560490.2834295062127315No Hit
GGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2547610.28200377440357777No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG2501730.27692515830078496No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC2360430.26128417191620273No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2314680.2562199459636575No Hit
ATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2140870.23698031508252348No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA2111970.2337812739890031No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2107810.2333207891810777No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1873270.2073587442650132No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1799250.1991652141009171No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1719700.1903595490839778No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1718010.19017247713075813No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1658500.18358510912122883No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1656610.1833758984753204No Hit
TGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1655510.18325413566553245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1651380.18279697166151032No Hit
TTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1639020.18142880045334728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1631280.18057203304629374No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1584840.17543142860397246No Hit
CATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1560160.17269951392618413No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACC1513290.16751131129458208No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1509390.167079606787152No Hit
GCATGGAATAATAGAATCTGTAGGCACTATCAATAGATCGGAAGAGCACA1498550.16587968964342326No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA1353710.14984684839825063No Hit
GCAGGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1320890.14621388892803133No Hit
GCATGGAATAATAGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACA1300130.14391589262694196No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA1295310.14338235013314377No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1286040.14235622172702148No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1265730.14010803748448175No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1244640.13777351234045598No Hit
GCATGGAATAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1244390.13774583897459508No Hit
TCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1234020.13659794775868483No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1217710.13479253736991956No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA1198300.13264397724447907No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG1153480.1276826962129364No Hit
GCATGGAATAATAGAATCTGTAGGCACCATTAATAGATCGGAAGAGCACA1134430.12557398573433562No Hit
ATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA1084590.12005702351630604No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1029950.11400873267374714No Hit
TCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1010120.11181368129366033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATTGGAAGAGCACACGT964970.1068158714191813No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG948660.10501046103041602No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA939800.10402971694430561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)