FastQCFastQC Report
Mon 24 Jul 2023
SRR6398772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150318347
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA108525487.219709514235145No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT80490265.354653081702661No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT67343154.480035294693601No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT47612273.1674290564145178No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA38274282.5462148010448784No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT37074692.466411501983853No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC35512602.3624927168737426No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27433931.8250553274112309Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT23893361.5895172130917592No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA21497391.4301241617565152No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC13403550.8916775807812736No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA12929800.8601611352205728No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC12156340.8087063384218827No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG11971940.7964390401392586No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG10303770.6854632322427016No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA10063230.6694611935827102No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG9727280.6471119589945996No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC9709390.6459218181796531No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG8887020.5912132602150022No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA8271020.5502335653012469No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA8246440.5485983690334221No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC7330170.48764306861357387No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG7201660.4790938793386279No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC7009540.46631300436000667No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA6548470.4356401018699334No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG5580740.3712614003132964No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA5513860.3668121762940887No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC5101550.33938305614816267No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA5093680.3388595006303522No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5010940.33335518251807283No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA4164500.2770453562797627No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC4161570.27685043662700737No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA4155460.2764439659518076No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG4077680.2712696142141584No Hit
TGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3884030.2583869552530404No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA3814670.25377274804651756No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3775670.2511782543750298No Hit
ATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3769060.250738520960452No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3612900.2403499021979No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC3526080.23457416013229576No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA3431320.22827020576536805No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG3175150.21122837387241888No Hit
TTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3018790.20082645001411573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA2934570.19522367419327727No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA2933280.19513785632568192No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA2865830.19065071278358325No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT2752670.18312268960754338No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA2734850.18193720557610973No Hit
TCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2728410.1815087814929205No Hit
ATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA2666200.17737023145950373No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2652680.17647080698672132No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2558490.17020477214268462No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG2547430.16946900034764217No Hit
CATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2484260.1652665858546196No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG2266140.1507560484283399No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC2177230.14484126811213538No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA2021840.13450387396822558No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1986390.13214554574632198No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA1967300.1308755743568681No Hit
TGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1938510.1289603058234801Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG1938180.12893835241549056No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1920290.12774821160054403No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1896930.1261941764168016No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1890610.12577373539106307No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT1876020.12480312865601163No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA1856940.12353382252134533No Hit
GGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1850260.12308943232325459No Hit
TCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1847670.1229171313332763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1842340.12256255053150632No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT1826150.12148550303044511No Hit
TAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAG1802870.1199367898850032No Hit
GCATGGAATAATAGAATCTGTAGGCACTATCAATAGATCGGAAGAGCACA1786730.1188630686578798No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1709670.11373661526493503No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1689590.1124007836515126No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1665960.11082878658850606No Hit
GCATGGAATAATAGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACA1655530.11013492584507997No Hit
AGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACA1601850.10656383814545273No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1549050.10305129286713084No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1548970.10304597082883037No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1523990.10138416436950308No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT1509670.10043151951371578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)