FastQCFastQC Report
Tue 4 Jul 2023
SRR6398773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140213374
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA90555606.458413874271366No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT84873956.053199318918037No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA65797474.692667191647495No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC64813314.622477025622391No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA56029753.99603464359969No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC49990123.5652890001776862No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA48015173.424435817370745No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA31247372.228558454060167No Hit
TCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG30085732.1457104370086695No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC28956382.0651653386502202No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA28874762.059344210631434No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT21968181.5667678034764358No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT19777581.4105344900979275No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT15998141.1409853100033098No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCA12799640.9128686968191779No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA12273070.8753137913933945No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT11347650.8093129546971747No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC11013250.7854635892293698No Hit
CCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG8879100.6332562826710098No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA8260920.5891677636970636No Hit
TCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC7917720.5646907833485271No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC7563750.5394456879698224No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT7353940.5244820654554678No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGG7220500.5149651416276453No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT6711010.47862837962946386No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA6364300.45390106652736284No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA6226840.44409743681084235No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT6130550.43723004625792683No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA5097690.3635666024269553No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA4968620.3543613464433143No Hit
CTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCA4918230.35076753805239724No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA4812760.3432454310670821No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGC4766440.3399418945585034No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4396820.31358064317031553No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT4135360.29493334922530284No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA3959980.2824252699318112No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGCTGTAGGC3926020.2800032470511693No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3861690.2754152396332749No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTACTGTAGGCAC3649300.2602676118470696No Hit
TTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCA3568620.25451352450872483No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACC3263770.23277166128246796No Hit
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC3261280.23259407479917002No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA3227080.23015493514905364No Hit
TACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCA3112490.22198239092370747No Hit
GCTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGG3093620.22063658492377483No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG2913440.20778617024079316No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC2850960.20333010458759804No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA2785650.19867220369434946No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGCTGTAGGCAC2767530.1973798875990246No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2732960.19491435959596834No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGC2629380.18752704717026494No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA2481060.17694888363502329No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA2459340.17539981599758095No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCAC2455370.17511667610252357No Hit
CCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC2402470.1713438548308523No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA2305600.1644350987517068No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2201050.15697860604937727No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA2018070.14392849572252644No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1926200.13737633900743307No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1864900.13300443080415425No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1860950.1327227173065531No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC1824590.13012952673116618No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1801060.12845137012393698No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC1774450.12655354830845164No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1773340.1264743832496321No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT1642720.11715858146313489No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATACTGTAGGCACC1627570.11607808539005701No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACCA1587130.11319390973360359No Hit
TTGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGCTGTAGG1551830.11067631822339573No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCA1516980.10819082065595255No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCAT1504360.10729076386108503No Hit
TTTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGC1496180.1067073673014958No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC1482360.10572172665925578No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1476210.10528310944147169No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA1429160.10192750942574137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)