FastQCFastQC Report
Wed 5 Jul 2023
SRR6398774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113043769
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA112304099.934567025980884No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA65830915.82348859935836No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT60868725.3845267667959655No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA45876094.0582590624698645No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA35870683.173167377319134No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT29056952.5704158890880575No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT21345511.888251797407781No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT19137571.6929345305179977No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT16334501.4449712836450102No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA14434311.2768779851988126No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC14282111.2634141736728541No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT13076821.1567926402029287No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT12535431.1089005710699544No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT10733030.9494579042211517No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT8972280.7936996509732439No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA7694070.6806275187091471No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA6421270.5680339621372674No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA6069440.5369106191071885No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC5649100.49972679166420925No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT5616690.49685976057645426No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA5600190.49540014894584766No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA5010490.4432345138810791No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGA4991740.4415758643008444No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC4467040.3951602144475561No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3951050.3495150626126063No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3950720.3494858703799941No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA3616220.31989556186860685No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC3564630.31533184283691035No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG3359310.29716896647350816No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC3337700.29525731754396833No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT3239900.286605801333464No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA3035480.26852254014991306No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2873730.2542139231044216No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC2749150.24319341298678745No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACC2727420.2412711486999341No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2530850.22388230880730808No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA2460600.217667901713362No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2384610.21094572669458678No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2356590.208467040761884No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCAT2327140.20586185515452868No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCA2307280.2041050135191441No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA2142070.18949032033777996No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA2065150.18268587629982508No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1982450.17537012588460316No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC1912490.16918137257083138No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1888800.1670857241145242No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1684410.1490051167703016No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA1680490.14865834843139386No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1551930.1372857622961952No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGCTGTAGGCAC1536670.13593584269116152No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1535970.1358739197734994No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1529910.13533784422916756No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1493830.13214616013024122No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT1453070.12854047709608832No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC1441110.12748247981717595No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1430760.1265669052488864No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1414210.12510287055273256No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACCA1374870.12162280258012274No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACTGTAGGCACCATC1323780.1171033142038992No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1315690.11638766219834726No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1273290.11263690261424317No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1262460.1116788666166996No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC1238890.10959383351770587No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1220230.10794314545545629No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1183690.10471076915349486No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAA1170950.10358377205204473No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAA1163060.10288581230868195No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA1148710.10161639249660899No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1146920.10145804675001592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)