FastQCFastQC Report
Tue 4 Jul 2023
SRR6398776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120496951
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA89736307.447184286015669No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA80480566.679053646759908No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT52326044.342519836871225No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACC49273764.089212182638546No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA39646283.290230970242558No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT39352023.2658104353196458No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA35560022.9511136758970773No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCA35206222.9217519371091805No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC19644251.6302694663203554No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT15404021.278374255295472No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT13443321.115656445116192No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT12637251.0487609765329249No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA12465161.0344792873638768No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT12084151.002859400151959No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT9002330.7471002316066901No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT8002560.664129667480134No Hit
TCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC6189730.5136835371046028No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCAC6035430.5008782338401243No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5360510.4448668580834049No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATC5211690.4325163381104971No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA5194170.43106235941189913No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA5176060.42955941681877086No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA5148010.4272315570872826No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA4567920.3790900900056799No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA4377190.363261473728078No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4279500.3551542146489665No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA3966980.3292182886851635No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACC3914480.32486133196847444No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT3780490.31374154853096653No Hit
TTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACC3462760.28737324648156454No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3436130.2851632320555563No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGC3199100.2654921949020934No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2946100.24449581301023957No Hit
TTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCA2724370.2260945175284975No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2603340.21605027997762366No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2543200.2110592823215917No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC2503570.20777040242287959No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGCTGTAGGCAC2477640.2056184807530939No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA2435920.2021561524822317No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCATCA2433300.20193871959465598No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCTCTGTAGGCACCA2171390.18020289990574118No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG2146210.17811322047476535No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA2120540.1759828761144338No Hit
CCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC2051570.1702590798334806No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2048770.17002670880859053No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1916890.15908203353626765No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGACTGTAGGCA1876180.15570352481366934No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGCTGTAGGCACCA1839870.15269017055875547No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1836260.15239057791595076No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1758050.14589995725286029No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1629440.13522665814174833No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1559250.12940161448566445No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1538620.12768953797013505No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1529240.12691109503675324No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1466420.12169768511404078No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1446480.12004287145821639No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1435910.11916567083925635No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT1423360.1181241507098383No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCACCAT1397360.11596641976443038No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1395450.11580790952959465No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1389420.1153074819295635No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1387130.11511743562706413No Hit
TGGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGAT1370220.11371408061603151No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTCTGTAGGCAC1311560.10884590764458431No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCACTGTAGGCACCATC1237590.1027071631048988No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCAT1225320.10168888007796978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)