FastQCFastQC Report
Tue 4 Jul 2023
SRR6398779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75278398
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT31858294.232062696126982No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA23383213.1062310863735436No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA21782972.8936548304335594No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA19500972.59051341661123No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA14318891.9021246971807237No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT12083621.6051909074898218No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG11094171.473752138030355No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6930530.9206532264408709No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG6240960.8290505863315528No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT6191740.8225121900176463No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA5705580.757930581891501No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT5436180.7221434228714592No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA5223920.6939467548180289No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA4337030.5761320797501562No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3651680.4850900254280119No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3367340.44731823331309467No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA3168070.4208471598983815No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT2834170.3764918057900223No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT2630980.3494999986583136No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG2406540.31968533655564774No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC2255830.2996649849004491No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2179380.2895093490166993No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2143180.2847005325485274No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2097680.27865630190483065Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG2094510.27823519836328076No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG2087630.2773212575538603No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA2078250.2760752161596213No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2021860.2685843553684551No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1961420.26055549162988295No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1770360.23517503653571376No Hit
TGGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGAT1608990.21373860798684902No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1605280.21324577071897838No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1457230.19357877408602664No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1385110.18399833641518248No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGATC1383680.18380837488066631No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1243450.16518018887702685No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1234230.1639554019202162No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1214030.1612720291948827No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTCTGTAGGCACCATCAATAGAT1139140.15132362407605965No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1134450.15070060337894015No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT1122800.1491530146536859No Hit
TGGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATC1092420.14511732834697144No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATC1087740.14449563605219123No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1078780.1433053875562017No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1061440.14100193789990056No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA1052890.13986615389982132No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1036420.13767827524703702No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATAG1029410.13674706520720592No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1025660.13624891432997818No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1022330.13580655635099992No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1006280.13367447059646512No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG989550.1314520534828597No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA987450.13117308899161217No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG967930.1285800476253493No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC904040.12009288507972765No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG895890.11901023717321933No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA829760.1102255125036003No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA822740.10929297406142996No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTTCTGTAGGCACCATCAATAGA811790.1078383734999249No Hit
TGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATCA800640.10635720489163439No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA796670.10582982916294259No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTGTAGGCACCAT795330.10565182324947989No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCACTGTAGGCACCATC779760.10358350080723025No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA775040.10295649490309292No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA768410.10207576415215425No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG767420.10194425232056613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)