FastQCFastQC Report
Mon 17 Jul 2023
SRR6398780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101893500
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA71000846.968142226933023No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT30328262.9764666048374036No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA23178472.274774151442437No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA22498032.207994621835544No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA11889411.1668467566625937No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT10190471.000109918689612No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT8031700.7882445887127245No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT7758610.7614430753679087No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT5944630.5834160177047604No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA4267190.41878922600558427No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA3737490.36680357432024613No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3526010.3460485703209724No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT3469820.3405339889198035No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA3254370.3193893624225294No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3202240.31427323627120474No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC3031220.29748904493417144No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2960360.29053472498245714No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2957330.29023735567038134No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2533790.24867042549328466No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT2269350.22271783774234863No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA2253210.2211338309116872No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2245450.22037225141937414No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATCG2121630.2082203477159976No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2118630.20792592265453635No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2110510.20712901215484797No Hit
TCGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAATAGATC2055490.20172925652764898No Hit
ATCACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTCTGTAGGCACCAT2036200.19983610338245322No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1885700.1850657794658148No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGA1860500.18259260894954044No Hit
TTTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGAT1842800.1808555010869192No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1795430.17620652936644635No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAA1772450.17395123339565333No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1704700.16730213409098715No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTGCTGTAGGCACCATCAATA1686220.16548847571238595No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT1667330.16363457924205174No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1552250.1523404338843989No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1502470.14745494069788556No Hit
AAATGAAAGTGAACGTAGGTTGGGGCCTCCTGTAGGCACCATCAATAGAT1478880.1451397782979287No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA1465460.14382271685632547No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1455540.14284915131976034No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA1443180.14163612006654006No Hit
TAGTCAAGGATGCTGGCATAATGGTTCTGTAGGCACCATCAATAGATCGG1441050.1414270782729026No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATCTGTAGGCACCATC1440130.14133678792072113No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1381120.13554544696177873No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1374460.13489182332533478No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCCTGTAGGCACCATCAATAG1367070.13416655625726862No Hit
TGTTTTCATGCCAGGTCGTACTGATAACCGCCTGTAGGCACCATCAATAG1315020.12905828144091624No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA1310540.1286186066824675No Hit
ACGATCAACTTAGAACTGGTACGGACCTGTAGGCACCATCAATAGATCGG1260140.12367226564991879No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1203310.11809487356897154No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1194780.11725772497755008No Hit
AAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAATAGATC1192930.11707616285631565No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAAT1190890.11687595381452204No Hit
TGGCGCCAGTGAAATACCACTACCTTTATCTGTAGGCACCATCAATAGAT1166890.11452055332283217No Hit
ATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTCTGTAGGCACCATCA1132630.11115821912094492No Hit
TCTACATGGTATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCG1108670.1088067442967412No Hit
ATTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1106890.10863205209360755No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTCTGTAGGCACCATCAATAG1097460.10770657598374773No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC1094770.10744257484530417No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1091270.10709907894026606No Hit
ATGCGAGTGTTTGGGTGTAAAACCCATCTGTAGGCACCATCAATAGATCG1073280.10533350998837021No Hit
TTCGAAGAGTCGAGTTGTTTGGGAATGCCTGTAGGCACCATCAATAGATC1066650.10468283060254088No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1064820.10450323131504953No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCCTGTAGGCACCATCAATAGA1023970.1004941433948191No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1019530.10005839430385648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)