FastQCFastQC Report
Tue 4 Jul 2023
SRR6441591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20512123
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT6553353.194866762450674No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC4438552.163866704582456No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA3914231.9082520127243776No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC1524950.7434384046936536No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT1034150.5041652685097491No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCC931710.4542240703217312No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA906310.44184114925597906No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAA659810.32166831292889575No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA640480.3122446174879119No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA626020.30519512777882624No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCC544530.2654674018871669No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC529160.25797427209265467No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT507240.247287908716226No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT491330.2395315199699222No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT459510.22401874247731454No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAA433700.2114359396148317No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC423930.2066729026537136No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT397530.1938024650105696No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC338670.16510723926528717No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT336380.16399082630305992No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA325970.15891577873241108No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC323880.15789686908566217No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAC322890.15741422767404428No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAA304810.1485999279548002No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAT279950.13648026584083958No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAA279020.1360268754238652No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTAAAAAAAAAAAAAAAAAAA273220.13319927927499264No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT272090.1326483855425399No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG249320.12154763307532818No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAA230490.11236769592304025No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAAAAA226730.11053463359204702No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAAA215540.10507932309103256No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA215020.10482581447078881No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA209820.10229072826835135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)