FastQCFastQC Report
Tue 4 Jul 2023
SRR6441592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124476
Total Bases56.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT257712.2918230357962286No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA207591.8461043188116064No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC164031.4587238856142772No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA138651.2330187571811226No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT135541.2053614305685494No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG83830.7455027941903607No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC83690.7442577698412416No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGTAT81130.7214916103144932No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC78970.7022826632137992No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT77650.6905438622078195No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTA50580.4498095112745848No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGT49320.4386042921325133No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTATG45340.4032100284932716No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG44890.3992081645139603No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTATG44750.39796314016484124No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCC36860.32779712506091724No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCG34320.3052088261554715No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCC30840.27426107804879785No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT29520.26252227704281816No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC28290.25158384883270074No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTC26140.2324638320426581No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGT23440.20845264816679057No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCC23330.2074744147496256No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT22560.2006267808294708No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTT22110.19662491685015954No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGT21890.1946684500158296No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCCCGTAT21220.18871011920218841No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT19810.17617094540034647No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCCCGT18990.16887865992693485No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAATCTCGTATGC18110.16105279258961508No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGT17330.1541162283588089No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA17250.15340478587359802No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC16950.15073687655405718No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA16930.15055901593275448No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTT16840.14975864313689222No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAATCTCG15390.13686374809244484No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTA15380.13677481778179346No Hit
TTCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC15360.13659695716049075No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT14980.13321760535573904No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC14650.1302829051042441No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTCGTATGC14270.12690355329949238No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAAATCTCGTA13680.12165666497106206No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCC13520.1202337800006403No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTA13050.11605405540002632No Hit
ACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATCTCGTAT12750.11338614608048549No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT12430.11054037613964192No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCCCGTAT12420.11045144582899057No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAACTCGTA12390.1101846548970365No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAATCC11530.10253664818101942No Hit
TAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGTA11280.1003133904147354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)