FastQCFastQC Report
Tue 4 Jul 2023
SRR6441593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804354
Total Bases92 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT412512.2861921773665257No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA393512.1808913328537525No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC239201.3256822109186999No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT209271.1598056700625266No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA179710.9959797245994965No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG144650.8016719557248744No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC135600.7515155008385273No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT131130.7267420916294696No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC118480.6566338977828076No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGTAT105720.5859160674679138No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT83200.46110685597172174No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGT79400.4400466870691671No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTATG76850.42591420530561075No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG74770.4143865339063177No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTATG69000.3824083300727019No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT62430.34599640647012725No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTA62240.3449433980249995No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCG54580.3024905312372184No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCT43160.23919918153533065No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC42590.23604015619994745No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTC41100.22778235313026157No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT38150.2114330114822258No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGT37640.2086065151295145No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCCCGTAT36730.2035631588923238No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGT33920.18798971820385577No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAATCTCG29870.16554401187350154No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGT28550.15822837425471942No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTA28250.15656572934135984No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA28010.15523561341067219No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAATCTCGTATGC26770.14836334776878596No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA26490.14681154584965034No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAC25360.14054891667599595No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAAATCTCGTA24840.1376669988261727No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTA24040.13323327905721383No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC23800.13190316312652617No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTCGTATGC23510.13029593971027859No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT23500.1302405182131666No Hit
TTCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC23160.1283561873113591No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAATCT22740.12602848443265569No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAACTCGTA22540.12492005449041596No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCCCGT21660.12004296274456122No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCC20740.11494418501025853No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC20580.11405744105646674No Hit
ACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAATCTCGTA20090.11134178769797944No Hit
ACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATCTCGTAT19350.10724059691169249No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG18880.10463578654742915No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT18840.10441410055898122No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCCCGTAT18570.1029177201369576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)