FastQCFastQC Report
Tue 4 Jul 2023
SRR6441594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825842
Total Bases93.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT408172.235516545243236No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA392882.1517743594462173No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC235081.287515568159786No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT206731.1322447396872237No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA183991.0076994613991792No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG145770.7983713815324655No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC135820.7438759761249878No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT129250.7078925777805527No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC118860.6509873252997794No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGTAT103270.5656020619527867No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT80350.44007093713475753No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGT79740.43673001278314333No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTATG78100.42774785550995104No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG76310.4179441594617716No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTATG68100.3729786038441442No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTA66690.3652561393592655No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT59260.324562585371571No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCG54680.2994782681086315No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCT43170.2364388594412879No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC41610.22789485618142205No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTC41430.2269090096514375No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT38310.2098210031317058No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCTCGT38060.20845177184006064No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCCCGTAT37560.2057133092567703No Hit
TCCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGT32870.1800265302255069No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAATCTCG30680.16803206411069524No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGTA28480.15598282874421773No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA28360.15532559772422805No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA27520.15072498058430028No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTCGT26930.14749359473601767No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAATCTCGTATGC26760.14656251745769897No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAC26640.14590528643770928No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAAATCTCGTA23480.12859820291131435No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAACTCGTA23380.12805051039465626No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAATCT23340.12783143338799305No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC23290.127557587129664No Hit
CCAACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAATCTCGTA23200.12706466386467177No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTCGTATGC23180.12695512536134013No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCCCGT23150.12679081760634273No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT22980.12585974032802402No Hit
TTCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC22850.1251477400563685No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCC20970.11485112074319684No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC20940.11468681298819941No Hit
ACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAATCTCGTA20460.1120578889082407No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT19380.10614280972833356No Hit
ACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATCTCGTAT19230.10532127095334645No Hit
AACGGGGCCTTTCCTTCTGGCAAAAAAAAAAAAAAAAAAAAATCCCGTAT19200.10515696319834902No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG18370.10061111531008708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)