FastQCFastQC Report
Tue 4 Jul 2023
SRR6441595.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441595.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12387336
Total Bases631.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT3443222.7796291309124093No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA2662872.1496712448907496No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC2146931.7331652261632364No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT786340.6347934697177827No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC752630.6075801931908523No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA532190.4296242549649093No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAA412320.33285607171711495No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT406260.32796397869566146No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA374740.30251863677549395No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAC347210.28029432639915475No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA295830.23881648160669894No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT278600.22490711481467848No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAA266220.21491303699197312No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC231250.1866825926090969No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA213310.17220005980301173No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC199940.16140677866492037No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCC197580.15950160712521239No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT197130.15913833289094603No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAA191910.15492435177345637No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAA184690.14909581850367182No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT175310.14152356890940876No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTAAAAAAAAAAAAAAAAAAA170420.13757598889704775No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTA168270.13584034533333075No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAA166080.13407241072656784No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAT159460.12872824310247175No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC156720.12651630665382774No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC143860.11613473631457158No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT141590.11430221962171688No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG139710.11278454059855969No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT139010.11221944734525649No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA135890.10970074598767644No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAAA135640.10949892696863958No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAAAAA135480.109369762796456No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAACC126340.10199125946046834No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA124960.10087721847538485No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAA124810.10075612706396274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)