FastQCFastQC Report
Tue 4 Jul 2023
SRR6441596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12605123
Total Bases642.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT3461672.7462405563198393No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA2730742.1663731484413122No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC2150641.7061634384686288No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCT791400.6278399663374963No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC764260.6063090379998671No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA551960.43788545339859036No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAA422190.3349352481526757No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCT414090.3285092894373185No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA388040.307843088877435No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAC366170.2904930003459704No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA309300.2453764235382709No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAT281730.22350436405896237No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAA270250.2143969559043573No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAATCTC231470.18363168689428894No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAAAAAAAAAAAAAAAAAAAAA218340.17321528714951848No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC202480.16063310131920172No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCC201980.1602364372009698No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCGTAT198960.15784058592684894No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAA195370.1549925375579437No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAAA191340.15179542476499436No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTTGAGTAAAAAAAAAAAAAAAAAAA179950.14275941615167104No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAT177240.14060949663085398No Hit
TTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAA167730.1330649451020827No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAT167650.1330014788431656No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCCCGTA166140.13180355320610515No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAATC160660.12745611447028324No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATT149490.11859463806898195No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATCTCG146390.116135320535944No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAATCTC145630.11553239107623148No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAAAAAAAAAAAAAAAAAAA141520.11227181202436501No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAATCTCGT139820.11092315402237646No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAAAAA139630.11077242165744833No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA138950.1102329584566529No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAACC131190.10407673134169336No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTTGAGTAAAAAAAAAAAAAAAAAAA126970.10072888618381591No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAACT126810.10060195366598168No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA126690.10050675427760601No Hit
TTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAAAA126570.10041155488923037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)