FastQCFastQC Report
Tue 4 Jul 2023
SRR6441597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16828365
Total Bases858.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCT910600.5411102029222684No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT567820.33741840042095594No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAAATC566010.33634283544479815No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG522560.31052333366907603No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC471660.28027678268209655No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTAAAAAAAAAAAAAAAAAAAA450260.26756015810210915No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA417090.24784939000312864No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA401900.2388229634905114No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAAAAAAAAAAAAAAAAAAA385910.22932114914312826No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAA385380.2290062047025959No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA338510.2011544199332496No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAATCT328930.1954616506119281No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC324390.19276382464963174No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC303910.1805938960796251No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA289400.17197154922655886No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA282890.16810308072115146No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCAAAAAAAAAAAAAAAAAAAA281770.16743753775247924No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA273080.1622736373973348No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGT270510.16074645397814938No Hit
TAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTAT254600.15129217841424286No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAAAAAA254390.15116738910761682No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA253750.1507870788398041No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA250340.1487607381941145No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTAAAAAAAAAAAAAAAAAAAA241170.14331160513811056No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA227980.13547364821240804No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTTAAAAAAAAAAAAAAAAAAA226590.1346476618495023No Hit
AAATGAAAGTGAACGTAGGTTGGGGCCTCAAAAAAAAAAAAAAAAAAAAA220610.13109413778462733No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA216780.12881821852568565No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA212250.12612633491132383No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTAAAAAAAAAAAAAAAAA207930.12355924060358804No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA204200.12134274482399211No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC199600.11860926477408827No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA199090.11830620502942503No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA197870.11758123858140704No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCGAAAAAAAAAAAAAAAAAAA195960.1164462501259035No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAAAAAAAAAAAAAAAAAAAA195790.11634523021101575No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCAAAAAAAAAAAAAAAAAAAA191160.11359392311730819No Hit
GGAATGCAGCTCTAAGTGGGTGGTAAAAAAAAAAAAAAAAAAAAATCTCG188630.11209050908986108No Hit
ACTAATAGGGAACGTGAGCTGGGTTCAAAAAAAAAAAAAAAAAAAAATCT187910.11166266003857178No Hit
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG185540.11025432357807785No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG184950.109903725049938No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA183690.10915498921018174No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT182310.10833494519521059No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAATCTC179900.10690283934297837No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAAAAAAAAAAAAAAAA176580.10492997982869993No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAAATC169440.10068714340341442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)