FastQCFastQC Report
Tue 4 Jul 2023
SRR6441598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17005621
Total Bases867.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCT920820.541479784831145No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAAATC568140.3340895342781072No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT559770.32916763227876245No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG528730.31091484398011693No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC467550.27493850415694904No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTAAAAAAAAAAAAAAAAAAAA456600.2684994567384513No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA416010.24463087822549967No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA397610.23381092639898302No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAAAAAAAAAAAAAAAAAAA394250.23183510910892344No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAA388200.22827746190509599No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA345760.20332100780089124No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAATCT326020.19171308122179131No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC320110.18823775973838297No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC301140.17708262462158836No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA293140.17237829774049415No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCAAAAAAAAAAAAAAAAAAAA290090.17058477311707698No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA280110.164716125332912No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA277800.16335775094599603No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGT269800.15865342406490185No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAAAAAA258290.15188507376472754No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA257360.15133819576480034No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA254570.14969756176501875No Hit
TAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTAT249490.14671031419552394No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTAAAAAAAAAAAAAAAAAAAA247210.14536958103441208No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTTAAAAAAAAAAAAAAAAAAA233110.13707820490648356No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA229470.13493773617558572No Hit
AAATGAAAGTGAACGTAGGTTGGGGCCTCAAAAAAAAAAAAAAAAAAAAA225570.1326443768210523No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA217240.12774599645611295No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTAAAAAAAAAAAAAAAAA214560.1261700469509464No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA210470.12376495983298699No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA206750.12157744783327817No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA203220.11950166359699536No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCGAAAAAAAAAAAAAAAAAAA198690.11683783850057579No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTC197910.1163791666296691No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAAAAAAAAAAAAAAAAAAAA196350.11546182288785573No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA194070.11412108972674388No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCAAAAAAAAAAAAAAAAAAAA191450.11258042267318553No Hit
GGAATGCAGCTCTAAGTGGGTGGTAAAAAAAAAAAAAAAAAAAAATCTCG190330.11192181690983234No Hit
ACTAATAGGGAACGTGAGCTGGGTTCAAAAAAAAAAAAAAAAAAAAATCT189580.11148078626472975No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA188440.11081041968417384No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCG185720.10921094854460182No Hit
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG182880.10754091250181337No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT180840.10634130914713436No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAAAAAAAAAAAAAAAA179410.10550041071713877No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAATCTC177690.10448898043770351No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAAATC177430.10433608981406796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)