FastQCFastQC Report
Tue 4 Jul 2023
SRR6441599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926750
Total Bases47.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCT36530.3941731858645805No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAAATC26660.28767197194496896No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTAAAAAAAAAAAAAAAAAAAA24430.2636093876449959No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT21540.2324251416239547No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA21080.22746155921230105No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA20490.2210952252495279No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG19970.2154842190450499No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAAAAAAAAAAAAAAAAAAA19960.21537631507957916No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAA19400.20933369301321825No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA16950.18289722147288912No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC16920.18257350957647694No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCAAAAAAAAAAAAAAAAAAAA16500.17804154302670622No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAAAAAA14520.1566765578635015No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA13390.14448340976530888No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAATCT13330.14383598597248448No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTAAAAAAAAAAAAAAAAAAAA12890.13908821149177233No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA12640.13639061235500405No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA12510.13498786080388456No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA12200.1316428378742919No Hit
AAATGAAAGTGAACGTAGGTTGGGGCCTCAAAAAAAAAAAAAAAAAAAAA12090.13045589425411383No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA11890.12829781494469922No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTTAAAAAAAAAAAAAAAAAAA11700.12624763960075533No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA11500.1240895602913407No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC11470.12376584839492852No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA11080.11955759374157002No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA11030.11901807391421634No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA10960.11826274615592124No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTAAAAAAAAAAAAAAAAA10860.11718370650121392No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA10780.11632047477744807No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCAAAAAAAAAAAAAAAAAAAA10520.11351497167520906No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC10490.11319125977879688No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAAAAAAAAAAAAAAAAAAAA10140.10941462098732128No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCGAAAAAAAAAAAAAAAAAAA10130.10930671702185056No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGT9810.10585379012678715No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCC9580.10337199892096036No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA9390.10132182357701647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)