FastQCFastQC Report
Tue 4 Jul 2023
SRR6441600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925769
Total Bases47.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCT38040.4109016396098811No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAAATC25650.2770669573079245No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTAAAAAAAAAAAAAAAAAAAA23120.2497383256514314No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA20260.21884508986583046No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT20250.21873707155888783No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAAAAAAAAAAAAAAAAAAA19850.21441633928118137No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA19850.21441633928118137No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG19710.21290408298398414No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAA19140.2067470394882525No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTAAAAAAAAAAAAAAAAAAAA16690.1802825542873006No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCAAAAAAAAAAAAAAAAAAAA16610.17941840783175933No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC16170.17466560232628225No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAAAAAA13540.1462567876003625No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAATCT13490.14571669606564921No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA13200.14258416516431205No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTAAAAAAAAAAAAAAAAAAAA12940.13977568918380287No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCAAAAAAAAAAAAAAAAAAAAA12810.13837145119354827No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA12320.13307855415335793No Hit
AAATGAAAGTGAACGTAGGTTGGGGCCTCAAAAAAAAAAAAAAAAAAAAA12040.1300540415589634No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAA11870.1282177303409382No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA11570.12497718113265836No Hit
TCGTCAATTAAAATGTTACGTGAGTTGGGTTAAAAAAAAAAAAAAAAAAA11530.12454510790488771No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAAAAAAAAAAAAAAAA11110.12000833901329598No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAAAAAAAAAAAAAAAAAAAA11030.11914419255775469No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTAAAAAAAAAAAAAAAAAAAAA10920.11795599118138542No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA10410.11244705752730971No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCAAAAAAAAAAAAAAAAAAAA10400.11233903922036707No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA10310.11136687445788312No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAA10240.1106107463092845No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCC10240.1106107463092845No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGCGAAAAAAAAAAAAAAAAAAA10190.1100706547745712No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATC10190.1100706547745712No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA10160.10974659985374322No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAATC9920.10715416048711936No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTAAAAAAAAAAAAAAAAA9890.10683010556629137No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAAAAAAAAAAAAAAAA9540.10304946482329826No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)