FastQCFastQC Report
Tue 4 Jul 2023
SRR6441621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11416574
Total Bases570.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA2289282.0052250351112337No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA404840.3546072578340928No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA404290.3541255020989659No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA359950.31528723065255826No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAAA351860.30820104174860163No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA298970.2618736584197676No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGGTAAAAAAAAAAAAAAAAAAAA297490.2605772975325172No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT281600.2466589363849435No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT275740.24152604800704658No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA260790.2284310512067806No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA257660.22568942311414966No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC245390.214941890623229No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC228910.20050673695979196No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT227140.19895635941220197No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA222560.19494464801787295No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA219270.19206287280229603No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATCTCG213920.18737670337878948No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATTAAAAAAAAAAAAAAAAAAA189940.16637215332725913No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATGAAAAAAAAAAAAAAAAAAA184720.16179985344114617No Hit
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA177510.15548447371339247RNA PCR Primer, Index 28 (96% over 29bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT164610.14418511192587197No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAAAAAAAAAAAAAAAAAAAAATC146510.1283309686426068No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC143720.12588715318623608No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAATCTCGT140540.12310172911768452No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAGAAAAAAAAAAAAAAAAAAAA134960.11821409820494308No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAAAAAA132610.11615568733667385No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAATCTC131720.11537611896528677No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA130560.11436005232392836No Hit
TCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA129560.11348413280551592RNA PCR Primer, Index 19 (96% over 28bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAA128790.11280967477633833No Hit
ACAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA127030.11126805642393243RNA PCR Primer, Index 28 (96% over 29bp)
TTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA119250.10445340257068364RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA117720.1031132457075126RNA PCR Primer, Index 19 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)