FastQCFastQC Report
Tue 4 Jul 2023
SRR6441622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24668300
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA1487090.6028344069108937RNA PCR Primer, Index 28 (96% over 29bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA1467780.5950065468637887No Hit
ATAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA1176920.47709813809626117RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA1111790.45069583230299615RNA PCR Primer, Index 19 (96% over 28bp)
ACAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA1090130.44191533263337973RNA PCR Primer, Index 28 (96% over 29bp)
TTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA851250.3450785015586806RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA847160.34342050323694784RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA819510.332211785976334RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA805450.3265121633837759RNA PCR Primer, Index 19 (96% over 28bp)
TTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA714610.2896875747416725RNA PCR Primer, Index 28 (96% over 29bp)
GTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA679270.2753614963333509RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA653660.2649797513407896RNA PCR Primer, Index 28 (96% over 29bp)
ATAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA640130.25949497938650007RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA627150.25423316564173454RNA PCR Primer, Index 19 (96% over 28bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAAATCTCGTATG585590.23738563257297826No Hit
ACAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA564730.22892943575357846RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA557470.22598638738786214RNA PCR Primer, Index 19 (96% over 28bp)
ACAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA551120.2234122335142673RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA542920.22008812929954638RNA PCR Primer, Index 28 (96% over 29bp)
GTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA512910.20792271863079337RNA PCR Primer, Index 28 (96% over 29bp)
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA510210.2068281965113121No Hit
TCAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA480010.19458576391563262RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCTC474020.19215754632463525No Hit
TTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA472590.19157785497987295RNA PCR Primer, Index 19 (96% over 28bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG466220.18899559353502268No Hit
GCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA430680.17458843941414692RNA PCR Primer, Index 28 (96% over 29bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAATCTCGTATGC421680.17094003234920932No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAATC395350.160266414791453No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATCC393200.1593948508814957No Hit
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGTATGC371640.1506548890681563No Hit
CCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA371340.15053327549932505RNA PCR Primer, Index 19 (96% over 28bp)
AGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA368600.14942253823733292RNA PCR Primer, Index 28 (96% over 29bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA352940.14307430994434153No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA342730.1389353948184512No Hit
TTAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA335820.13613422894970467RNA PCR Primer, Index 19 (96% over 28bp)
CTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA327560.1327858020212175RNA PCR Primer, Index 19 (96% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC327370.13270878009429105No Hit
ATAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA326280.13226691746087085RNA PCR Primer, Index 19 (96% over 27bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC316160.12816448640562988No Hit
CCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA310880.12602408759419984RNA PCR Primer, Index 28 (96% over 29bp)
ATAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA310480.12586193616909153RNA PCR Primer, Index 19 (96% over 28bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT310410.12583355966969753No Hit
AGAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA302820.12275673637826684RNA PCR Primer, Index 28 (96% over 29bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATCT287290.11646120729843563No Hit
CTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA286890.1162990558733273RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA286150.11599907573687689RNA PCR Primer, Index 19 (96% over 27bp)
GTAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA272410.1104291742844055RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA271140.10991434350968651RNA PCR Primer, Index 19 (96% over 28bp)
TGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA269390.10920493102483755RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA261760.10611189259089601Illumina PCR Primer Index 12 (96% over 25bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCTCG260890.10575921324128537No Hit
TTAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA257170.10425120498777783Illumina PCR Primer Index 12 (96% over 25bp)
ATAAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAA252090.10219188188890195RNA PCR Primer, Index 19 (96% over 28bp)
TGAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA249790.10125951119452901RNA PCR Primer, Index 19 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)