FastQCFastQC Report
Tue 4 Jul 2023
SRR6441623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12453693
Total Bases622.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA760030.6102848367949972RNA PCR Primer, Index 28 (96% over 29bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA706180.5670446509320568No Hit
ATAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA563220.4522513924182971RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA557380.44756202035813797RNA PCR Primer, Index 19 (96% over 28bp)
ACAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA543530.43644082120861655RNA PCR Primer, Index 28 (96% over 29bp)
TTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA467380.3752943002529451RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA402020.3228118759632183RNA PCR Primer, Index 28 (96% over 29bp)
ATAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA398950.3203467437329634RNA PCR Primer, Index 19 (96% over 28bp)
ACAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA377840.30339594849495644RNA PCR Primer, Index 19 (96% over 28bp)
TTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA356550.2863006178167392RNA PCR Primer, Index 28 (96% over 29bp)
GTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA345700.2775883426707243RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA315660.25346698364894654RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA303740.24389552560834765RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA266280.21381609455123068RNA PCR Primer, Index 19 (96% over 28bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAAATCTCGTATG265330.213053268616787No Hit
ACAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA264370.21228241293566497RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA259590.20844419402341138RNA PCR Primer, Index 19 (96% over 28bp)
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA256240.20575422888616252No Hit
GCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA250810.2013940764398159RNA PCR Primer, Index 28 (96% over 29bp)
GTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA248970.19991660305099862RNA PCR Primer, Index 28 (96% over 29bp)
TTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA245730.19731496512721164RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCTC231350.18576818940373752No Hit
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG229080.18394543690775098No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATCC226460.18184164327802202No Hit
ACAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA219230.17603613642957153RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA202350.16248192403650868RNA PCR Primer, Index 28 (96% over 29bp)
AGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA199050.1598321076326516RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA198640.15950288801883908RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAATC197810.15883641904453563No Hit
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAATCTCGTATGC177430.1424717953140486No Hit
CCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA175350.1408016080049508RNA PCR Primer, Index 19 (96% over 28bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGTATGC172180.13825617830791234No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA171050.13734881693325826No Hit
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTTTTCTGCTTGAAAA168120.13499610115650032RNA PCR Primer, Index 19 (96% over 27bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA167870.13479535748954144No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC161380.1295840518952892No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC153740.12344932543302617No Hit
CTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA151460.12161854319036128RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA151280.12147400775015091RNA PCR Primer, Index 19 (96% over 28bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT147800.11867965590608345No Hit
TTAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA147560.11848694198580292RNA PCR Primer, Index 19 (96% over 28bp)
AGAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA143910.11555608444820344RNA PCR Primer, Index 28 (96% over 29bp)
TGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA142980.11480931800711644RNA PCR Primer, Index 19 (96% over 28bp)
CCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA138550.11125214022860527RNA PCR Primer, Index 28 (96% over 29bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCTCG127740.10257198406930378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)