FastQCFastQC Report
Tue 4 Jul 2023
SRR6441624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374283
Total Bases886 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA947740.5454843805640786No Hit
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA749550.43141348624285675RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA604130.3477150682995091RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA579270.33340656417303666RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA513670.29564961040406673RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA420630.24209919914393013RNA PCR Primer, Index 19 (96% over 28bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAAATCTCGTATG410470.236251475816297No Hit
ATAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA408130.2349046576483185RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATCT398180.2291778026178116No Hit
GCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA397840.228982111089131RNA PCR Primer, Index 19 (96% over 28bp)
TCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA390590.22480927702167625RNA PCR Primer, Index 28 (96% over 29bp)
TTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA390130.22454451789463772RNA PCR Primer, Index 19 (96% over 28bp)
ACAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA388340.22351425955246615RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA350490.20172918790375408RNA PCR Primer, Index 28 (96% over 29bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG334570.1925662198549431No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCTC316160.18197009914020626No Hit
GTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA307060.17673247293140096RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA305470.17581732725315918RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA299760.17253086069796375RNA PCR Primer, Index 28 (96% over 29bp)
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA296800.17082719327180293No Hit
TCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA294520.1695149089030034RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAATC275800.15874036355917534No Hit
TTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA273090.15718058696292675RNA PCR Primer, Index 28 (96% over 29bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAATCTCGTATGC269010.1548322886187591No Hit
GCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA262830.15127530730332872RNA PCR Primer, Index 28 (96% over 29bp)
ATAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA255370.14698160493874768RNA PCR Primer, Index 19 (96% over 28bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGTATGC243640.14023024719926572No Hit
ACAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA237130.13648332998835117RNA PCR Primer, Index 19 (96% over 28bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA233770.13454943723433077No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC228550.13154499670576333No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAATCT226720.1304917158308058No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA222830.12825277451737144No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT217210.12501810866094445No Hit
TTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA214390.12339502009953446RNA PCR Primer, Index 19 (96% over 28bp)
CCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA208080.1197632155525497RNA PCR Primer, Index 19 (96% over 28bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC205160.11808257065917484No Hit
AGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA204150.11750125170632941RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA198490.11424356331711646RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCTCG178030.10246753779709931No Hit
CTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA175820.10119554286067518RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA175290.10089049430126124RNA PCR Primer, Index 19 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)