FastQCFastQC Report
Tue 4 Jul 2023
SRR6441625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17519170
Total Bases893.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA941960.5376738738193647No Hit
ATAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA714250.40769625501664747RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA587870.3355581343180071RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA569050.32481561626492583RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA486420.27765013981826764RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA415720.2372943467070643RNA PCR Primer, Index 19 (96% over 28bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAAATCTCGTATG398330.2273680773689621No Hit
GCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA398040.22720254441277754RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA394380.22511340434506885RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAAATCT391570.2235094470799701No Hit
TCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA379570.21665980751371214RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA377060.2152270912377698RNA PCR Primer, Index 19 (96% over 28bp)
TTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA367620.20983870811231353RNA PCR Primer, Index 19 (96% over 28bp)
GTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA338570.19325687232899733RNA PCR Primer, Index 28 (96% over 29bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCGTATG331360.18914138055627064No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAAATCTC311630.17787943150274813No Hit
GTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA304160.17361553087275253RNA PCR Primer, Index 28 (96% over 29bp)
ACAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA296810.16942012663841952RNA PCR Primer, Index 19 (96% over 28bp)
GCAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA295780.1688321992423157RNA PCR Primer, Index 28 (96% over 29bp)
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAA295180.1684897172640028No Hit
TCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA289710.16536742322838355RNA PCR Primer, Index 19 (96% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAAATC280100.15988200354240528No Hit
GCAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA263340.1503153402815316RNA PCR Primer, Index 28 (96% over 29bp)
ACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAAAAATCTCGTATGC258150.14735287116912502No Hit
TTAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA257310.14687339639948696RNA PCR Primer, Index 28 (96% over 29bp)
ATAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA241810.1380259452930704RNA PCR Primer, Index 19 (96% over 28bp)
AGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGTATGC238980.13641057196202788No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA236180.134812322729901No Hit
ACAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA228500.1304285534074959RNA PCR Primer, Index 19 (96% over 28bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC226440.1292526986152883No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAAAAAAAAATCT226320.1291842022196257No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA221600.12649001065689758No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT212790.12146123360866981No Hit
CCAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA208250.11886978663943554RNA PCR Primer, Index 19 (96% over 28bp)
TTAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA205690.11740853019863383RNA PCR Primer, Index 19 (96% over 28bp)
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC203120.11594156572486025No Hit
AGAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA202160.11539359455955962RNA PCR Primer, Index 28 (96% over 29bp)
TCAAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAA192160.10968556158767796RNA PCR Primer, Index 19 (96% over 28bp)
ATAAAAAAAAAAAAAAAAAAAAATCTCGTTTGCCGTCTTCTGCTTGAAAA180920.10326973252728297RNA PCR Primer, Index 19 (96% over 27bp)
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAAAAAAATCTCG178430.10184843231728444No Hit
CTAAAAAAAAAAAAAAAAAAAAATCTCGTATGCCGTCTTCTGCTTGAAAA176320.1006440373602174RNA PCR Primer, Index 19 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)