FastQCFastQC Report
Tue 4 Jul 2023
SRR6441627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24434194
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA1545370.6324620325106692No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG534380.21870170957961615No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA491460.2011361618885403No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGT408270.16708961220492888No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA391810.1603531509981463No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT366470.1499824385449342No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCT329420.1348192618917571No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT273230.11182280045742454No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA270440.11068095800499905No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC250920.10269215346329821No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAT247540.10130884611949958No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAATCTCGTA244810.10019155941873917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)