FastQCFastQC Report
Tue 4 Jul 2023
SRR6441628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16416233
Total Bases820.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA1147870.6992286232779469No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAAATCTCG355940.2168219712768453No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA343760.20940248594181138No Hit
AAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTCGT296110.1803763384693675No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA274090.16696278616415838No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT219810.1338979533246147No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA199350.12143467992931144No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAAT183090.11152984975298535No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAAAAAAAAAAAAAAAAAAAATCTC176070.10725359465840914No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAATC170130.10363522496299853No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC166320.10131435147149774No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA165930.10107678174402131No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC164190.10001685526758788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)