FastQCFastQC Report
Tue 4 Jul 2023
SRR6441676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13532815
Total Bases690.1 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA1311500.969125787945819No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA1256320.9283508272299592No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA1069200.7900795215186197No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAA959610.7090985873966356No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA925450.6838562412920002No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAA866310.6401550601260714No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAA838190.6193759391523493No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA779360.5759038307994309No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA699500.5168917183897068No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA665700.49191539232598686No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA636840.47058945237927213No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA607700.44905660795628993No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA586540.43342054110693157No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT559690.4135798797219942No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAA416440.30772607177442385No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA409190.3023687237282118No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA401940.29701137568199965No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA372010.2748947650581199No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAA368680.2724340796796528No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAA360720.2665520809971909No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA346480.2560295104898722No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAA339820.25110813973293805No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC331700.24510790992118048No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA329720.243644799696146No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAA325800.24074813702840095No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCAAAAAAAAAAAAAAA320340.23671349974118466No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA308570.22801612229236856No Hit
ATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCT286150.21144898529980644No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAT269470.19912339007072807No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAATCTCGTATG262260.19379559980683989No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAA261260.19305665524874166No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA257700.190426012621912No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA252510.18659089036538223No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA250030.18475830786129863No Hit
AACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCTCGTAT239770.17717673669521086No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAA234140.17301647883311788No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAA222540.1644447219591785No Hit
GAAAAGAAACCAACCGGGATTGCCTTAGTAACGAAAAAAAAAAAAAAAAA217220.16051353691009593No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAATCTCGTA190640.14087239055584516No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAATCTCGTATGC186800.138034843452748No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAA184350.13622442928540734No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAA182700.1350051707645453No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAAAAAAAAAAAAA181190.13388936448181699No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAATC179670.1327661687535077No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA179330.13251492760375427No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAA178380.13181293027356097No Hit
ATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAATCTC173080.1278965241156404No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAT171880.12700979064592252No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA167350.12366237179773758No Hit
AACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAATCTCGTATG159980.11821635040455368No Hit
AATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCTCG158920.11743306917296956No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA157100.1160881900772308No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAATCTC156870.11591823282886819No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAATCT151200.11172841718445128No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA149480.11045743254452234No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA147300.1088465334078682No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA147230.10879480728880134No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAA143540.1060681018694189No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAATCTCGTAT137650.10171571842222035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)