FastQCFastQC Report
Tue 4 Jul 2023
SRR6441677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13760054
Total Bases701.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA1334850.9700906697023136No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA1270550.9233612019255157No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA1048260.7618138707885884No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAA943540.6857095182911346No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA938800.6822647643679305No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAA854390.6209205283642055No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAA824500.5991982298906676No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA798680.5804337686465475No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA721830.5245836971279328No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA672140.4884719202410107No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA623210.4529124667679356No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA616840.4482831244702964No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA601950.4374619460068979No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT570170.4143661064120824No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAA420980.30594356679123497No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA418690.3042793291363537No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA411630.29914853531824803No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA386460.28085645594123393No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAA362820.2636762908052541No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAA358450.26050043117563343No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA358070.2602242694687099No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAAAAAAAAAAAAA354160.25738271085273356No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA337780.24547868780166127No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC337000.24491182956113397No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCAAAAAAAAAAAAAAA326250.23709936022053404No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA318450.23143077781526147No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAA317760.23092932629479507No Hit
ATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCT288510.20967214227502304No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAT269050.19552975591520205No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAA268910.1954280121284408No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA265460.1929207545261087No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAATCTCGTATG263970.19183790993843483No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA252350.18339317563724677No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA243350.17685250363116306No Hit
AACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCTCGTAT239710.17420716517536922No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAA239390.17397460794848624No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAA213770.155355494971168No Hit
GAAAAGAAACCAACCGGGATTGCCTTAGTAACGAAAAAAAAAAAAAAAAA210590.1530444575290184No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAATCTCGTA195920.142383162159102No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAATCTCGTATGC189020.13736864695443782No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA182970.13297186188368157No Hit
GTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTAAAAAAAAAAAAAAAA181820.13213610934957087No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAAAAAAAAAAAAA181510.13191081953602798No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAA180530.13119861302869887No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAATC178490.1297160607073199No Hit
ATAATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAATCTC175900.12783380065223582No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAT174020.12646752694429833No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA172500.12536288011660418No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAATCTC164560.11959255392457034No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA163860.11908383499076385No Hit
AATAACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAAATCTCG159380.11582803381440217No Hit
AACTTTTCGAATCGCATGGCCAAAAAAAAAAAAAAAAAAAATCTCGTATG158850.11544286090737726No Hit
TTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAA151680.11023212554253058No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAATCT151590.11016671882246973No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA148350.10781207690027961No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA144960.10534842377798807No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAA142970.10390220852330957No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAT141110.10255046964205228No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAATCTCGTAT138730.10082082526711016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)