FastQCFastQC Report
Tue 4 Jul 2023
SRR6441678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13314650
Total Bases679 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA841430.6319580311912066No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA723550.5434239728419448No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA706900.5309189501789382No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA667170.5010796378425268No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA666600.500651537967577No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA616930.4633467646539714No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA543880.40848238594330305No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA490460.3683611660839752No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA458540.34438757308678786No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA419250.3148787238117412No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA381320.28639130581727645No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA373710.2806757969604909No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA335580.2520381684835876No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA296150.222424171870834No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAATCTCGTATG289490.2174221628056314No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAA277370.20831940756985723No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA256020.19228443856954558No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAA252460.18961069198213998No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA243120.18259586245226123No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAA226200.1698880556379627No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAAA224160.1683559087170898No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAATCTCGTATGC216040.16225736313008604No Hit
TTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAA213290.16019196899655644No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAATCTC211690.15899028513704827No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATCTCG205960.1546867548151848No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAT201360.1512319137190989No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA189780.14253472678590876No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT182960.13741254933475533No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA179210.1345961027890331No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA178720.13422808710705875No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT175480.1317946772915548No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAT168840.126807689274596No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA162850.12230888532556243No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAATCTCGTA161470.12127243299673666No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAAAAAAAAAAAAA160960.12088939626651846No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAATCTCG160720.12070914368759224No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA159050.11945488615923064No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGCCAAAAAAAAAAAAAAAAAAAAA157020.11793024976247968No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA142880.10731036865407652No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAAAAAA140480.10550784286481431No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAAATCT133150.10000262868344266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)