FastQCFastQC Report
Tue 4 Jul 2023
SRR6441680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14148062
Total Bases721.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA935300.6610799415495918No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA807860.5710040004065575No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA728480.5148973760505149No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA702430.4964849602722973No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA692430.4894168544073386No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA496150.35068407248992833No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA485440.3431141311085575No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA413830.29249942500958787No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA402790.28469623613467343No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA395520.2795577231708484No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA392660.27753624489347023No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA384030.27143646953201084No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA381640.2697471922302857No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA367340.25963980084339466No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAT310570.21951416384802386No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATCTCG308450.21801572540465258No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA303940.2148280096595562No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA303180.21429083361381934No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA259570.18346682393673425No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT258060.18239953995112548No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA249100.17606651709612242No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA246100.1739460853366348No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAATCTCGTA240320.16986072014668863No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA228250.16132951636768342No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAA221540.15658681733229612No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA221500.15655854490883628No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA217750.15390800520947676No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAAA211920.1497872994902058No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT210890.14905928458611503No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT205880.14551816354777072No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAATCTCGT202280.14297364543638555No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAA199080.14071185155959878No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAAATCTC187520.13254112117970646No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAATCTCGTAT180790.1277842859325892No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA174300.12319708522623098No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAA172990.12227116335792139No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA168440.11905517518936515No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA164640.11636929496068084No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAA164120.11600175345570299No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAATCTCG162960.11518185317536778No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAAA162010.11451038311819667No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA160080.11314623868625964No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAATCTCGTATG155990.11025538338749151No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGCCAAAAAAAAAAAAAAAAAAAAA155070.1096051176479153No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA153860.1087498768382553No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA153640.10859437850922621No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAAATCT145270.10267837390025573No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAAATCT142250.10054380592903821No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)