FastQCFastQC Report
Tue 4 Jul 2023
SRR6441681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14404796
Total Bases734.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA967110.6713805596413861No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA835770.5802025936361751No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA739520.5133845699723898No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA724170.5027283968478277No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA699840.4858381888920884No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA510290.3542500706014858No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA483340.33554102397562585No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA415930.2887441099478257No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA411980.28600196767798725No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA402350.27931669424544436No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA400590.2780948789555923No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA392400.27240927257838293No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA375640.2607742587954734No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA372030.2582681490248109No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAT321890.22346029752868418No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATCTCG310980.2158864311580671No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA308900.21444246763369645No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA307100.21319288381452953No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA262930.18252948531863972No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT259900.1804260192230421No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA239350.1661599372875534No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAA236160.16394539707469652No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA228750.1588012770191261No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAA228290.1584819389320057No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA223410.15509417835559766No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT215660.14971402580085133No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAA208970.14506973927294772No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT208100.14446577376035036No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAAA207200.14384098185076694No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAATCTCGT206970.14368131280720672No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAAATCTC192300.13349720468099652No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAATCTCGTAT183460.12736035970242135No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA179150.12436830066874949No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAA174830.12136929950274894No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA172170.11952269230331343No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAAAAAAAA171970.11938384965673933No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAA168860.11722484650251207No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA164020.11386485445541887No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA163510.11351080570665494No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAA158300.10989395476339962No Hit
ATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAAATCTCGTATG157430.1092899892508023No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA157370.10924833645683006No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAATCTCG157230.10915114660422821No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGCCAAAAAAAAAAAAAAAAAAAAA156710.10879015572313555No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAAATCT151590.1052357839708386No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAATCTC144300.10017496950321268No Hit
TGCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAAATCT144070.10001530045965247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)