FastQCFastQC Report
Tue 4 Jul 2023
SRR6441682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6441682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5681709
Total Bases289.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA635041.1176918775671194No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA363000.638892276953994No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA317910.5595323519736756No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA305570.5378135346248814No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAA295900.5207940075776496No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA283550.4990575898906473No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAA259850.4573447883374527No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA257900.4539127223868734No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAA253710.4465381806776799No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA252740.4448309478714943No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAA249590.4392868413359431No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA234680.4130447370676675No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA232430.4090846609708453No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATCTCG175510.3089035358903457No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAAAAAAA158050.2781733453790048No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA151980.2674899400866887No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAA148680.261681828478016No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAA139180.24496150718032197No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT134120.23605573604702387No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAA129060.2271499649137258No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT129040.22711476423730959No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAA121500.21384410922840294No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAA112770.1984790139727325No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAAAT111050.19545175580093946No Hit
AGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAATCTCGT103930.18292031499677297No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAAAAA103540.1822339018066571No Hit
AGAGGGTGAGAATCCCGTGTGGCAAAAAAAAAAAAAAAAAAAAATCTCGT99450.17503536347954463No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAT96140.16920965153266385No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA93060.16378874736456936No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA92210.16229271861688094No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAAATCTC87800.15453096946710926No Hit
GCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAAA84150.14810684602115315No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCAAAAAAAAAAAAAAAAAAAAATCT82720.14558999765739497No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTTAAAAAAAAAAAAAAAAAAAA79990.14078510532658395No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACAAAAAAAAAAAAAAAAAAAA76710.1350121943943275No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAATC73940.13013690071068407No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAT72940.12837686688987415No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAAAAAAAAAAAAAAAAAAAAATC72460.1275320506558854No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC71600.12601842156998888No Hit
TTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAATCTCGTA70440.1239767823378494No Hit
GCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAATCTCG69640.12256875528120148No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA68070.11980550218252993No Hit
AGAGGGTGAGAATCCCGTGTGGCGAAAAAAAAAAAAAAAAAAAAATCTCG67950.11959429812403274No Hit
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAA67530.11885508391929259No Hit
AGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAATCTCGT64550.11361018313327909No Hit
TGCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAAATCT64450.1134341797511981No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAAA63660.11204375303275828No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGCCAAAAAAAAAAAAAAAAAAAAA62570.11012531616807548No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA62310.10966770737466491No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA61540.10831248133264129No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAA60550.10657004785003948No Hit
AGAGGGTGAGAATCCCGTGTGGCAAAAAAAAAAAAAAAAAAAATCTCGTA59810.10526762282264016No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGAAAAAAAAAAAAAAAAAAAAAT59020.10387719610420033No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAA58010.10209956194518234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)