FastQCFastQC Report
Tue 4 Jul 2023
SRR6466829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6466829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44131367
Total Bases999.7 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG12093882.740427233989829No Hit
TTACTTACTCCGTGAATCGGA8802411.994592644274989No Hit
TTTTTACTTACTCCGTGAATCGGA8468821.9190024183932484No Hit
GCGGGTGCACCGCCGACCGACCTTG7845521.7777650078231204No Hit
TCGTTCGAATTGTAGTCTG6397791.4497148932640134No Hit
TTTTACTTACTCCGTGAATCGGA5596681.2681864126257407No Hit
CGTTCGAATTGTAGTCT5171111.1717538683993178No Hit
ACGCATTTATTAGATA4394080.9956818242226668No Hit
TTTACTTACTCCGTGAATCGGA4391140.9950156313988643No Hit
TTACTTACTCCGTGAATCGG3574140.8098865371652775No Hit
ATTTTACTTACTCCGTGAATCGGA3498470.7927400028193098No Hit
TTACTTACTCCGTGAATCG3210300.727441776276724No Hit
TGTGCTTGCTAAGTACATG2475740.5609932726534395No Hit
TTATTTTACTTACTCCGTGAATCGGA2462040.5578889047329987No Hit
CGAGGCAAACTCTGAATACTAGAT2418890.548111278764603No Hit
CTGATGATTCATGATAACTCG2358930.5345245706982066No Hit
TCGAGGCAAACTCTGAATACTAGAT2336130.5293581773707576No Hit
ACTTACTCCGTGAATCG2268790.5140991893588974No Hit
TACTTACTCCGTGAATCGGA2158240.48904897960672733No Hit
TCGTTCGAATTGTAGTCT2043280.46299948061885327No Hit
GCGTTCGAATTGTAGTCTG1830650.4148183309164205No Hit
TTTACTTACTCCGTGAATCGG1830560.41479793725854897No Hit
TTTTTACTTACTCCGTGAATCGGAG1806050.4092440644315414No Hit
CTGATGATTCATGATAACTC1797010.40719563479644766No Hit
TTTTACTTACTCCGTGAATCGG1787770.4051018859216393No Hit
TTTATTTTACTTACTCCGTGAATCGGA1655180.37505749595293525No Hit
CGGGTGCACCGCCGACCGACCTTG1647150.3732379284783995No Hit
TTTTTACTTACTCCGTGAATCGG1602650.3631543976419312No Hit
TGAGGCAAACTCTGAATACTAGAT1565110.35464797634752626No Hit
TACTTACTCCGTGAATCGG1430780.3242093089933063No Hit
TGCGGGTGCACCGCCGACCGACCTTG1345340.3048489297872871No Hit
AGGCTCCCAAAGGCACGT1333100.3020753923167619No Hit
TCGGGTGCACCGCCGACCGACCTTG1285650.29132340269450524No Hit
TCTGATGATTCATGATAACTCG1246750.2825088105700419No Hit
TACTTACTCCGTGAATCG1205910.2732546218203483No Hit
CCAGATTGAGGTTCTGGTC1160800.2630328673027509No Hit
TTTACTTACTCCGTGAATCG1158780.2625751429816348No Hit
TTTTACTTACTCCGTGAATCGGAG1112640.2521199943795079No Hit
ACTTACTCCGTGAATCGG1090950.24720512283247423No Hit
ACTTACTCCGTGAATCGGA1070840.24264827327918487No Hit
CCGACCCTCAGTAGGAGCTT1030380.23348019108494872No Hit
ATTTTACTTACTCCGTGAATCGGAG963200.21825745846486017No Hit
TCTGATGATTCATGATAACTC958670.2172309776853275No Hit
CGACCCTCAGTAGGAGCTT944100.2139294710721288No Hit
CCGTTCGAATTGTAGTCTG921910.20890130142580898No Hit
TTTACGATAGGTGTCAAGTGGA908710.20591023160465438No Hit
AAGCCATCTCGTACTAAACGA902710.2045506544132204No Hit
TCCGATGTCGTCCAGCGG811840.18395985784895355No Hit
TTTTTTACTTACTCCGTGAATCGGA784660.17780097317175786No Hit
TCGACCCTCAGTAGGAGCTT774790.17556446869184905No Hit
GCGGGTGCACCGCCGACCGACCTT759770.17216099378929275No Hit
TTTTACTTACTCCGTGAATCG747280.16933080726912447No Hit
TTTGCTTGCTAAGTAAATG743080.16837910323512073No Hit
GCGTCTGTAGTCCAACGG728850.16515463932943658No Hit
TTACTTACTCCGTGAATCGGAG728720.16512518182362218No Hit
GCGTTCGAATTGTAGTCT721720.16353900843361593No Hit
CTACTTACTCCGTGAATCGGA684880.1551912044782116No Hit
CCAGATTTAGGCTCTGGTC675520.15307026405957466No Hit
GTCGTTGTAGTATAGTGG656260.14870602127507176No Hit
ATTTTACTTACTCCGTGAATCGG647210.14665532567799228No Hit
TTGGGCCAACCTATGGTGTACCGGT627020.1420803484288171No Hit
TTTACTTACTCCGTGAATCGGAG616210.13963084352225028No Hit
TTACTTACTCCGTGAATC596080.13506946204498946No Hit
CCAGACTCAAGTTCTGGTC581500.131765689469805No Hit
TATTTTACTTACTCCGTGAATCGGA578460.13107683702614514No Hit
TACTTACTCCGTGAATCGGAGTAGTCTG575540.13041517612631398No Hit
TCCGTTGTAGTCTAGCTG554990.12575862424565276No Hit
AAGCCATCTCGTACTAAACGAA515680.1168511276797748No Hit
TTATTTTACTTACTCCGTGAATCGG502930.1139620261479777No Hit
CTGTTTTTGGTCCCAAGGCT499960.11328903543821792No Hit
TCTGATGATTCATG498960.11306243923964558No Hit
TCGCGGGTGCACCGCCGACCGACCTTG482930.10943010217653126No Hit
TGTGAACCATCGAGT479180.10858036643188505No Hit
TTATTTTACTTACTCCGTGAATCGGAG477600.1082223444381408No Hit
GTACTTACTCCGTGAATCGGA467600.10595638245241759No Hit
TGCGTTCGAATTGTAGTCTG445580.10096673415985506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)