FastQCFastQC Report
Tue 4 Jul 2023
SRR6466830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6466830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26813632
Total Bases613.1 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG6526372.43397462902452No Hit
GCGGGTGCACCGCCGACCGACCTTG6083902.268957819664266No Hit
CGTTCGAATTGTAGTCT4457121.6622589584283098No Hit
TCGTTCGAATTGTAGTCTG3992541.4889963433525155No Hit
TTTTTACTTACTCCGTGAATCGGA2395490.893385125894172No Hit
TTACTTACTCCGTGAATCGGA2371850.8845687148984517No Hit
TCGTTCGAATTGTAGTCT1808810.6745859717922585No Hit
TGTGCTTGCTAAGTACATG1667120.6217434475120714No Hit
TTACTTACTCCGTGAATCG1660410.6192409890610866No Hit
CTGATGATTCATGATAACTCG1655760.6175067965428928No Hit
TTTTACTTACTCCGTGAATCGGA1627240.6068704157646378No Hit
CGGGTGCACCGCCGACCGACCTTG1603610.5980577342151933No Hit
CGAGGCAAACTCTGAATACTAGAT1500100.5594542358155732No Hit
TCGAGGCAAACTCTGAATACTAGAT1470220.5483106503438251No Hit
ACTTACTCCGTGAATCG1280790.47766374954351576No Hit
TTTACTTACTCCGTGAATCGGA1265520.47196888508054413No Hit
GCGGGTGCACCGCCGACCGACCTT1215390.453273170900533No Hit
TTACTTACTCCGTGAATCGG1190900.4441397569713793No Hit
TCGGGTGCACCGCCGACCGACCTTG1086280.40512229003515826No Hit
ACGCATTTATTAGATA1081510.4033433441616563No Hit
CTGATGATTCATGATAACTC1080230.4028659750383685No Hit
TGCGGGTGCACCGCCGACCGACCTTG1079170.4024706537331459No Hit
GCGTTCGAATTGTAGTCTG1075050.40093412186756344No Hit
ATTTTACTTACTCCGTGAATCGGA946620.35303684334893537No Hit
AGGCTCCCAAAGGCACGT915610.34147183044803475No Hit
TGAGGCAAACTCTGAATACTAGAT890200.33199530746151806No Hit
TCTGATGATTCATGATAACTCG857800.31991190152829724No Hit
CCGACCCTCAGTAGGAGCTT823370.30707141800111226No Hit
TTTACGATAGGTGTCAAGTGGA786770.2934216446321036No Hit
CGACCCTCAGTAGGAGCTT719060.2681695638994374No Hit
TTATTTTACTTACTCCGTGAATCGGA707450.2638396767733666No Hit
TTTACTTACTCCGTGAATCGG656070.24467778180889485No Hit
CCGTTCGAATTGTAGTCTG647680.24154877638359473No Hit
TTTTACTTACTCCGTGAATCGG643010.23980712497284964No Hit
TCGACCCTCAGTAGGAGCTT629340.23470897191398762No Hit
TTGGGCCAACCTATGGTGTACCGGT628760.23449266402999785No Hit
TTTACTTACTCCGTGAATCG607220.22645943675217145No Hit
TTTTTACTTACTCCGTGAATCGG602890.2248445865147996No Hit
TACTTACTCCGTGAATCG572570.21353690540692138No Hit
TACTTACTCCGTGAATCGGA570730.21285068729219528No Hit
TCTGATGATTCATGATAACTC562180.2096620107264842No Hit
GCGTTCGAATTGTAGTCT538250.20073744578876893No Hit
TTACTTACTCCGTGAATC531740.1983095762632977No Hit
TTTGCTTGCTAAGTAAATG524310.19553859768046342No Hit
CGTTCGAATTGTAGTC497680.1856070822483131No Hit
TTTATTTTACTTACTCCGTGAATCGGA480600.17923718800944236No Hit
CGGGTGCACCGCCGACCGACCTT478400.17841670982879157No Hit
TCCGATGTCGTCCAGCGG421410.1571625955036602No Hit
TACTTACTCCGTGAATCGG414650.15464148982129688No Hit
GCGTCTGTAGTCCAACGG398880.14876015304454093No Hit
TTTTACTTACTCCGTGAATCG386490.14413936910896666No Hit
ACTTACTCCGTGAATC370450.1381573372827672No Hit
TACTTACTCCGTGAATC370110.13803053610939392No Hit
GCGGGTGCACCGCCGACCGACCT358070.13354028279346863No Hit
ACTTACTCCGTGAATCGG357580.13335753992596006No Hit
CCAGATTGAGGTTCTGGTC349590.13037771235168738No Hit
TCGCGGGTGCACCGCCGACCGACCTTG346520.12923277234505195No Hit
TGGCTCCCAAAGGCACGT332190.12388847583199472No Hit
AGGCTCCCAAAGGCACG331620.12367589739428063No Hit
CTGATGATTCATGATAACTCGA331120.12348942508049636No Hit
TTTGGGCCAACCTATGGTGTACCGGT329270.12279947751949456No Hit
ACATAGTAAGGATTGACAGA320790.11963690707771331No Hit
CTGTTTTTGGTCCCAAGGCT317210.11830176531101792No Hit
CGCGGGTGCACCGCCGACCGACCTTG309340.11536669109205348No Hit
TCGGGTGCACCGCCGACCGACCTT299770.11179761100622251No Hit
CCGTTCGAATTGTAGTCT288130.10745653554132466No Hit
CTGATGATTCATGATAACT270950.10104934683969706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)