FastQCFastQC Report
Tue 4 Jul 2023
SRR6466831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6466831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29951648
Total Bases682.8 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG9257373.0907714994513826No Hit
GCGGGTGCACCGCCGACCGACCTTG8225652.746309652143348No Hit
CGTTCGAATTGTAGTCT6590822.2004865975989034No Hit
TCGTTCGAATTGTAGTCTG5143701.717334552008624No Hit
TTACTTACTCCGTGAATCGGA4349831.4522840279105844No Hit
TTTTTACTTACTCCGTGAATCGGA3022791.009223265444359No Hit
TCGTTCGAATTGTAGTCT2068590.6906431325581818No Hit
TTACTTACTCCGTGAATCGG2059170.6874980635456186No Hit
TTTACTTACTCCGTGAATCGGA2038760.6806837473517316No Hit
TTTTACTTACTCCGTGAATCGGA1912260.6384490095503259No Hit
CGAGGCAAACTCTGAATACTAGAT1876330.6264530085289464No Hit
CTGATGATTCATGATAACTCG1762260.5883682927897657No Hit
TCGAGGCAAACTCTGAATACTAGAT1719980.5742522080921891No Hit
TTACTTACTCCGTGAATCG1688990.5639055320094574No Hit
CGGGTGCACCGCCGACCGACCTTG1581690.5280811259534033No Hit
GCGTTCGAATTGTAGTCTG1554920.5191433873688687No Hit
ATTTTACTTACTCCGTGAATCGGA1459170.48717519650337776No Hit
TGCGGGTGCACCGCCGACCGACCTTG1413770.47201743289718145No Hit
TACTTACTCCGTGAATCGGA1167450.3897782185474402No Hit
GCGGGTGCACCGCCGACCGACCTT1135250.37902755801617327No Hit
TCGGGTGCACCGCCGACCGACCTTG1101810.36786289689301904No Hit
ACGCATTTATTAGATA1013360.33833196757654205No Hit
TTATTTTACTTACTCCGTGAATCGGA1009710.3371133368020351No Hit
TGTGCTTGCTAAGTACATG959100.3202161029670221No Hit
TCTGATGATTCATGATAACTCG941770.31443011082395195No Hit
CTGATGATTCATGATAACTC904240.30189991549045986No Hit
GCGTTCGAATTGTAGTCT859670.287019265183672No Hit
CCGTTCGAATTGTAGTCTG832480.2779413005922078No Hit
TGAGGCAAACTCTGAATACTAGAT776710.25932129010063154No Hit
ACTTACTCCGTGAATCG768050.25642996338632185No Hit
TTTTTACTTACTCCGTGAATCGGAG764530.255254735899674No Hit
CCGACCCTCAGTAGGAGCTT755550.25225657032294185No Hit
TTTTTACTTACTCCGTGAATCGG751070.25076082624902646No Hit
AGGCTCCCAAAGGCACGT704470.23520241690874572No Hit
TACTTACTCCGTGAATCGG699650.2335931565435064No Hit
TTTACTTACTCCGTGAATCGG693470.2315298310129713No Hit
TTTTACTTACTCCGTGAATCGG689510.23020770009049252No Hit
TTTATTTTACTTACTCCGTGAATCGGA675530.22554017728840828No Hit
TCGCGGGTGCACCGCCGACCGACCTTG661160.22074244462274664No Hit
TACTTACTCCGTGAATCGGAGTAGTCTG630640.210552688119198No Hit
CGTTCGAATTGTAGTC568590.18983596495257957No Hit
TACTTACTCCGTGAATCG556790.18589628190074883No Hit
CGCGGGTGCACCGCCGACCGACCTTG536570.1791454012814253No Hit
ATTTTACTTACTCCGTGAATCGGAG507530.16944977451658086No Hit
GCGGGTGCACCGCCGACCGACCT473720.15816158095875057No Hit
ACTTACTCCGTGAATCGGA456690.15247575024920162No Hit
TCTGATGATTCATGATAACTC451880.15086982859841302No Hit
ACTTACTCCGTGAATCGG444750.14848932519506106No Hit
TTGGGCCAACCTATGGTGTACCGGT432850.14451625499872328No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATT429580.14342449537334306No Hit
CTACTTACTCCGTGAATCGGA428470.14305389806931493No Hit
CGACCCTCAGTAGGAGCTT420020.14023268435847003No Hit
CTGTTTTTGGTCCCAAGGCT419860.14017926492725874No Hit
TTACTTACTCCGTGAATCGGAG406820.13582558128354072No Hit
TTTACTTACTCCGTGAATCG395950.13219639867562546No Hit
TGCGTTCGAATTGTAGTCTG380970.12719500442847084No Hit
TTTTACTTACTCCGTGAATCGGAG370160.12358585410725981No Hit
TTTACGATAGGTGTCAAGTGGA369890.1234957088170908No Hit
CCAGATTGAGGTTCTGGTC367210.12260093334430211No Hit
ATTTTACTTACTCCGTGAATCGG361210.1205977046738797No Hit
TACTTACTCCGTGAATCGGAGTAGTCT348090.11621731131455605No Hit
TTACTTACTCCGTGAATC342040.11419738907188011No Hit
CCGTTCGAATTGTAGTCT337500.11268161271126051No Hit
CTGATGATTCATGATAACTCGA330060.11019760915993672No Hit
CTGATGATTCATGATAACT325020.10851489707678187No Hit
TTTGGGCCAACCTATGGTGTACCGGT308340.10294592137300759No Hit
TTTGCTTGCTAAGTAAATG306760.10241840448979636No Hit
TCGACCCTCAGTAGGAGCTT303120.1012031124297401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)