FastQCFastQC Report
Tue 4 Jul 2023
SRR6466832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6466832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29972785
Total Bases683.7 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTCGAATTGTAGTCTG9902243.3037437128381626No Hit
GCGGGTGCACCGCCGACCGACCTTG8726632.9115178986537287No Hit
TCGTTCGAATTGTAGTCTG5446221.817055038429028No Hit
CGTTCGAATTGTAGTCT5167451.7240473316043206No Hit
TTACTTACTCCGTGAATCGGA4678371.56087263829504No Hit
TTTTTACTTACTCCGTGAATCGGA2684480.8956391606585774No Hit
CGAGGCAAACTCTGAATACTAGAT2605590.8693186168719389No Hit
TCGAGGCAAACTCTGAATACTAGAT2254330.7521256366400386No Hit
CGGGTGCACCGCCGACCGACCTTG2015510.6724466878870282No Hit
TTACTTACTCCGTGAATCG1991340.6643827058446521No Hit
TCGTTCGAATTGTAGTCT1940460.6474073063280573No Hit
TTTACTTACTCCGTGAATCGGA1936170.645976007901835No Hit
TTACTTACTCCGTGAATCGG1908130.6366208545518877No Hit
TTTTACTTACTCCGTGAATCGGA1779460.5936919108451216No Hit
GCGTTCGAATTGTAGTCTG1730260.5772770198031314No Hit
CTGATGATTCATGATAACTCG1598020.5331569955878307No Hit
ACTTACTCCGTGAATCG1413060.47144768162184464No Hit
TGCGGGTGCACCGCCGACCGACCTTG1405220.4688319754070234No Hit
TACTTACTCCGTGAATCGGA1327630.4429451584162099No Hit
ATTTTACTTACTCCGTGAATCGGA1293880.4316849435246007No Hit
TCGGGTGCACCGCCGACCGACCTTG1272820.42465856943223657No Hit
TGTGCTTGCTAAGTACATG1262810.4213188731043845No Hit
AGGCTCCCAAAGGCACGT1050050.3503344784276803No Hit
TGAGGCAAACTCTGAATACTAGAT1033930.34495626615945096No Hit
TTATTTTACTTACTCCGTGAATCGGA952990.3179517685793963No Hit
GCGGGTGCACCGCCGACCGACCTT947170.3160100070780877No Hit
CCGACCCTCAGTAGGAGCTT888090.29629879238782786No Hit
TCTGATGATTCATGATAACTCG858290.2863564396835329No Hit
ACGCATTTATTAGATA849160.28331034303285463No Hit
CCGTTCGAATTGTAGTCTG843170.281311863412092No Hit
TACTTACTCCGTGAATCG733750.2448054126435031No Hit
TACTTACTCCGTGAATCGG715550.23873323750195385No Hit
CTGATGATTCATGATAACTC689600.23007538338529437No Hit
TTTATTTTACTTACTCCGTGAATCGGA664840.22181455610481307No Hit
CGACCCTCAGTAGGAGCTT655260.21861832325558No Hit
GCGTTCGAATTGTAGTCT616380.20564655570044624No Hit
TCGCGGGTGCACCGCCGACCGACCTTG614850.20513609262535998No Hit
TTGGGCCAACCTATGGTGTACCGGT574260.191593807515718No Hit
TTTACTTACTCCGTGAATCGG545790.18209519068715171No Hit
ACTTACTCCGTGAATCGG544450.1816481184514552No Hit
TTACTTACTCCGTGAATC537990.17949282991220203No Hit
CGCGGGTGCACCGCCGACCGACCTTG527450.17597630650605206No Hit
TCGACCCTCAGTAGGAGCTT526550.1756760341089425No Hit
TTACTTACTCCGTGAATCGGAG514090.17151892958895879No Hit
TGAATGCAGCCCCAATCGGGCGGTA508940.16980070420549842No Hit
AAGCCATCTCGTACTAAACGA505960.16880646893506893No Hit
CTACTTACTCCGTGAATCGGA489210.1632180659888629No Hit
TTTTACTTACTCCGTGAATCGG471790.15740612692480863No Hit
ACTTACTCCGTGAATCGGA465450.15529087470517006No Hit
TTTTTACTTACTCCGTGAATCGG455680.1520312510165472No Hit
AAATGCAGCCCCAATCGGGCGGTA452370.15092691586717752No Hit
TACTTACTCCGTGAATCGGAGTAGTCTG444690.14836459141184244No Hit
TTTTTACTTACTCCGTGAATCGGAG443480.14796089185572847No Hit
TGCGTTCGAATTGTAGTCTG429240.14320991526146135No Hit
TACTTACTCCGTGAATC410030.1368007677631558No Hit
AGGCTCCCAAAGGCACG401530.13396486179045425No Hit
CTGTTTTTGGTCCCAAGGCT390390.13024815678623125No Hit
TTTACGATAGGTGTCAAGTGGA389850.1300679933479655No Hit
TTTACTTACTCCGTGAATCG385330.12855995864248182No Hit
TTTGGGCCAACCTATGGTGTACCGGT375460.12526697135418013No Hit
TTTGCTTGCTAAGTAAATG369430.12325514629354596No Hit
ACTTACTCCGTGAATC361920.12074953995766494No Hit
TCCGATGTCGTCCAGCGG357910.11941165961054337No Hit
CGGGTGCACCGCCGACCGACCTT351120.11714627119235001No Hit
GCGTCTGTAGTCCAACGG350790.11703617131340982No Hit
CTGATGATTCATGATAACTCGA350590.11696944411405212No Hit
TCTGATGATTCATGATAACTC350510.11694275323430907No Hit
CGTTCGAATTGTAGTC348290.11620208132143876No Hit
CGTTCGAATTGTAGTCTGG323340.10787786320156768No Hit
CCGTTCGAATTGTAGTCT302000.10075807103010281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)