FastQCFastQC Report
Tue 27 Jun 2023
SRR648666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR648666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148313823
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA54590643.6807519957192394No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA41625252.8065657777562647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA18826871.2693941548523093No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAAAAAAAAAAA18678151.2593667685310763No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAA18618111.2553185956240909No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA15872711.0702111023056833No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA15715381.0596031901894942No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA13597140.9167817082026131No Hit
CATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAAA12324750.8309913230407392No Hit
ATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAAAA12093920.8154277029188304No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA11139820.751097893282678No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAA10374640.6995059388361934No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA9750550.6574269210227289No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA9261440.6244488755441224No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA7979900.5380415553039853No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAA7941760.5354699811089085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7854980.5296188744322233No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAAAAAA7380500.49762725083285053No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAA6120290.412658097283353No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA5984310.40348970034977794No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA5935840.4002216300499516No Hit
ACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA5766780.38882282739080903No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAAAAAAAAAAAA5414260.36505430785099513No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAAA4872180.3285047813783345No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAAAAA4817240.3248004739248074No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA4740950.31965665128866644No Hit
TCCTCCCACTTATTCTACACCTCTCACGCGCAAAAAAAAA4573530.3083684249714202No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAAA4477530.3018956634945618No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAA4426070.29842599364457084No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA4183940.28210047555715695No Hit
AACGATGCCGACTGGCGATGCGGCGGCAAAAAAAAAAAAA3982000.26848475209219036No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA3807180.2566975837444363No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAA3739840.25215721126681495No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA3664460.24707474501550675No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAAAAAAAAAA3638950.24535474350222908No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGAAAAAAAAAAAA3613850.24366238607442542No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAAAAAAAAAAAAA3340440.2252278265391352No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCAAAAAAAAAAA3211920.21656241711199095No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA3086310.20809321326711402No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAA2920740.19692972245749474No Hit
TATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAAA2802090.18892979381969002No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGAGAAAAAAAAAAA2755060.18575881494201654No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA2690030.18137419328743215No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAA2624220.17693698044584827No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAA2367690.15964054813690562No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAA2339990.15777288675243709No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAAAAAA2202370.14849391347696567No Hit
TCATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA2142020.1444248389443781No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCAAAAAAAAAAA2140720.14433718696604564No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAA2138200.1441672769772781No Hit
ACGATGCCGACTGGCGATGCGGCGGCGAAAAAAAAAAAAA2078850.14016562704340782No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAAA2066150.1393093346396984No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAA2065640.13927494809435262No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA1992100.1343165431046842No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA1987650.1340165036403923No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAAAAAAA1980450.13353104652962794No Hit
GTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAA1973660.13307323350433764No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGAAAAAAAAAAAA1893970.12770016723255795No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAAAAAA1878930.12668610126785013No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA1864080.1256848459768986No Hit
GGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAAA1861920.1255392088436693No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAAAAAAAAA1842490.12422914889059261No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGTAAAAAAAAAA1834110.12366413075334186No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAA1828720.12330071216625574No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1826780.12316990844474422No Hit
TCACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAA1808750.12195424292987175No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAA1778400.11990790635880244No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAAAAAA1768340.11922961489570666No Hit
TTTGGTGGTTCAGTGGTAGAATTCTCGCAAAAAAAAAAAA1763170.11888102972033834No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAAAAAA1756290.11841714848116347No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAAAAAA1740080.11732419573595644No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGCAAAAAAAAAA1714400.11559273204089682No Hit
CCGTGGCGCAATGAAGGTGAAGGGCAAAAAAAAAAAAAAA1691750.11406556487995054No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA1687730.1137945179931071No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA1680840.11332996250794505No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAAAAAAAAAA1610180.10856574036258237No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAAAAAAAA1609190.10849899000985228No Hit
TGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA1605370.10824142804275229No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAA1604510.10818344288785545No Hit
CATTGATCATCGACACTTCGAACACAAAAAAAAAAAAAAA1601010.10794745679234498No Hit
TTCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAA1599170.10782339553070519No Hit
CCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAAAAAAAAA1554220.10479265981836365No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA1549360.1044649762685977No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCAAAAAAAAAA1549330.10446295353063617No Hit
CCCTACCTACTATCCAGCGAAACCACAGAAAAAAAAAAAA1544960.10416830803424168No Hit
ATTGATCATCGACACTTCGAACACAAAAAAAAAAAAAAAA1538860.10375701798206631No Hit
TCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAA1531780.103279651823148No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTAAAAAAAAAAA1523420.10271598217787158No Hit
CCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAAAAAAAAAA1518780.10240313203982343No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1504480.10143896027816639No Hit
GTCTAGCGGTTAGGATTCCTGGAAAAAAAAAAAAAAAAAA1492750.10064806973521273No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAA1485770.10017744603616616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)