FastQCFastQC Report
Mon 26 Jun 2023
SRR649752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16526261
Total Bases594.9 Mbp
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA6003933.6329633182000456No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAA2236331.3531977983404715No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA1869571.1312722218292448No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAA1747471.057389811282782No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAA1183960.716411292306227No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAA1124790.680607670422245No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA1076470.6513693569283457No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1050630.6357336363016414No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAA932900.5644955020376357No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA928030.5615486769814418No Hit
ATGTACACGGAGTCGACCCGCAACGCGTAAAAAAAA812540.4916659612237759No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACA787460.4764901147331511No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAA705000.4265937709685209No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAA550180.3329125686687388No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAA508680.307801020448606No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAA475340.28762706821585354No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC468460.28346399708923875No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAA439950.26621266601078125No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAA431450.2610693368572601No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAA422600.25571422356212337No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACAT416900.2522651675415268No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAA396100.2396791385540867No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAA375200.22703259981189938No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAA374780.2267784588419607No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAA357250.2161710988347576No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAA356970.21600167152146513No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAA356070.21545708372873937No Hit
ATGTACACGGAGTCGACCCGCAACGCCAAAAAAAAA351220.21252236062349492No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAA333800.20198156134651388No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAA326600.19762485900470772No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAA318410.19266911009090318No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAA302570.18308436493892963No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAA298190.18043403768099753No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAA284580.17219866005988893No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAA252320.15267821317840738No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAA251190.1519944529497628No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAA250530.15159508856843057No Hit
GGGGGGGGATGCGTGCATTTATCAGTAAAAAAAAAA246140.14893871033502373No Hit
ACCTGCGGAAGGATCATTAAAAAAAAAAAAAAAAAA243590.14739571158896742No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAA242060.14646991234133358No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA239530.1449390155462267No Hit
TTATAACCTAGACTTACAAGTCAAAGTAAAAAAAAA237710.1438377380098257No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA234260.14175015147104358No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAA232730.14082435222340975No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAA232230.14052180344967322No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAA226290.13692752401768313No Hit
GGGGGGGGGATGCGTGCATTTATCAGTAAAAAAAAA224530.1358625523341305No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAA223410.13518484308096065No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAA211810.12816571153027295No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAA210260.12722781033168967No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAA208230.1259994623103193No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAA206550.12498289843056454No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAA201650.12201792044794645No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAA201430.12188479898750239No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAA196910.11914975807292406No Hit
TGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAA195470.11827841760456283No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAA195400.1182360607762397No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAA185810.11243317529597288No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCAAAAAAA183320.11092648240276491No Hit
GGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAA181460.10980100096446499No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA179620.10868762147711451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176740.10694494054039204No Hit
CTAAACCATTCGTATGCCGTCTTCTGCTTGAAAAAA173030.10470002863926693Illumina PCR Primer Index 1 (95% over 22bp)
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAA172410.1043248681598336No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAA168780.10212836406250635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)