FastQCFastQC Report
Thu 29 Jun 2023
SRR649754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49598303
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA35861677.230422782811742No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAA8358251.6851887049441996No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAA5674301.1440512390111413No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA4467800.900796948637537No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAA4006390.8077675560794892No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAA3655470.7370151353767084No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAA3204360.6460624267729482No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAAAA3127660.6305981880065534No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAA3110180.6270738738783058No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAA2918780.5884838438928042No Hit
ACCTGCGGAAGGATCATTAAAAAAAAAAAAAAAAAA2661700.536651425352194No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAA2578710.5199189980350739No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAA2381240.48010513585515213No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA2160760.4356520020453119No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAA2159640.4354261878677583No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2111900.4258008585495354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2103320.42407096065363364No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAA2076110.4185848858578891No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTAAA2030170.40932247218216317No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCAAAAA1948750.3929065879532209No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTCAAAAAAAA1834660.3699037848129602No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAA1717340.34624974971421907No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAA1712830.345340444409963No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAA1591970.3209726752143113No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAA1581820.31892623423023164No Hit
AACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAAAA1559820.3144905985997142No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAA1553760.31326878260330804No Hit
ACGTTGAACGCACGTTCGTAAAAAAAAAAAAAAAAA1531670.30881500119066574No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAA1497670.3019599279435024No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAA1486730.29975420731632696No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAA1474690.2973267049076256No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAAAAA1454850.2933265680481044No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAA1383240.27888857407077017No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA1374670.2771606923728822No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAA1341620.27049715793703666No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA1308810.2638820122535241No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAAAAAAA1247970.2516154635371295No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAA1182570.23842952852640947No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAA1139690.2297840714429282No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAAAAAAAAAA1106480.22308827783886076No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAA1082050.2181627060909725No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAA1076070.2169570196786773No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAA1062110.2141424072513126No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAA1062040.2141282938652155No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTAAAA1056960.21310406527416872No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAA1050190.2117390992187777No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAAAAAAAAA999130.2014443921599495No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAA981240.1978374139131333No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAA972390.19605307867085697No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAA964430.1944481850518152No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAAAAA894390.18032673416265876No Hit
ATGTACACGGAGTCGACCCGCAACGCGTAAAAAAAA884260.17828432557460686No Hit
AAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAA866390.17468137972381836No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAA861240.1736430377466745No Hit
TCTCAACCTATTCTCAAACTTTAAATGGGTAAAAAA845410.17045139629071582No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTAAAAAAAAA842800.16992516860909535No Hit
GGGGGGGGGATGCGTGCATTTATCAGTAAAAAAAAA834530.16825777285162358No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAA834260.16820333550524902No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAA827520.1668444180438996No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAA826470.1666327172524431No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAA825440.1664250488570143No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAA824570.1662496396298075No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAA790330.15934617763031128No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAAAAAA754100.1520414922260546No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAA752020.1516221230391693No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAA752000.15161809064314155No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAA737100.14861395560247292No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAA730770.14733770225969223No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAAA729240.14702922396356988No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACA729170.14701511057747277No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAA717510.14466422369329854No Hit
GGGGGGGGATGCGTGCATTTATCAGTAAAAAAAAAA705090.14216010576007007No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAA702540.14164597526653283No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAA690920.13930315317441405No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAA678140.1367264521126862No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATAAAAA656180.13229888127422423No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAAAAAA652220.1315004668607311No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAA650150.1310831138718597No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAAAAAA637250.12848221843396537No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAA635160.1280608330490662No Hit
AGTTATGGTTCCTTTGGTCGCTCAAAAAAAAAAAAA624870.12598616529279236No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAA610940.12317760145946928No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAA599470.1208650223375586No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAA597680.12050412289307559No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAA594230.11980853457828991No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAA593300.11962102816299985No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC591100.11917746459994812No Hit
TCCTGCGGAAGGATCATTAAAAAAAAAAAAAAAAAA579130.11676407557734383No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAA576700.11627413945997306No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGTTAAAAAA574830.11589711043137907No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAA571650.1152559594629679No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTTAAAAAAA571410.115207570710635No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCAAAAAAAAA566380.11419342310965759No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGAAAAA565710.1140583378427282No Hit
AGCCCTCGACACAAGGGTTTGTAAAAAAAAAAAAAA565170.11394946314997915No Hit
ATGTACACGGAGTCGACCCGCAACGTGAAAAAAAAA558780.11266111261911521No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTTAAAAA556890.1122800511944935No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAA554240.11174575872081752No Hit
CTAAACCATTCGTAGACGACCTGCTTAAAAAAAAAA549800.11085056680265855No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATAAAA549100.11070943294168754No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAA549000.11068927096154883No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACA545920.11006828197327638No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCAA541030.10908236114449318No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAA534970.10786054514808703No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAA522920.10543102654137178No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAAAAAAA522070.10525964971019272No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAAAA519010.10464269311794801No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAA517530.10434429581189501No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTAAAAAAAA509200.10266480286634No Hit
ATGTACACGGAGTCGACCCGCAACGCTAAAAAAAAA507760.10237447035234248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)