FastQCFastQC Report
Tue 27 Jun 2023
SRR649755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92846591
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA26485462.8526044644977864No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA24827812.674068022594389No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA23754322.5584482687145726No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA18629172.006446310990567No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAA11519511.2407036032157603No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA11166391.2026709736709666No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAAA8378760.9024305480424155No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA6456850.6954321026175317No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAA6419540.6914136459786661No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA6276420.6759989712492513No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA6096240.6565927660176559No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA5778660.622387956063998No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAA5491570.5914670577404398No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA5222870.5625268460314283No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA5137160.5532954893303514No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA4586450.49398151839522036No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA4505950.4853113023826583No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAA4298780.462998151434553No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAA4130270.4448488582634122No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA3883580.4182792236281459No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA3842430.413847181529799No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA3788760.40806667850626854No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA3769820.4060267543910147No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA3638250.3918560671764459No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA3396310.3657980291381942No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA3360710.36196374727425373No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAAAAAAAAAA3343660.3601273847523384No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAA3209610.34568959026185464No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAA3056770.3292280273381281No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCAAAAAAAAAAA2907190.3131175812367737No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAAAAAA2827670.30455291568001674No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAA2739520.29505875988489444No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA2732540.2943069821486499No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA2671920.2877779325252771No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA2654850.28593941591242694No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA2653460.2857897065924585No Hit
TTCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAA2609380.2810420901721637No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAA2564140.2761695364776505No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA2529120.2723977232508192No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA2454670.2643791197460335No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAA2436790.2624533624503241No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA2416860.26030681083379786No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAA2323710.2502741323049761No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAA2316910.24954174138714474No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA2315810.24942326638573084No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA2309770.24877273092342184No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA2262010.24362876177112416No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAA2180450.23484437893901783No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA2177940.23457404052670064No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAA2150000.2315647754907878No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAA2095470.22569164655706098No Hit
ACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA2087840.22486986086543556No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA2061390.22202107560416515No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA2035170.21919706238864495No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAA1965960.2117428307087764No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAA1959930.21109337229193478No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTAAAAAAAAAAAA1918030.20658055178353288No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA1842830.19848116986869233No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA1756490.18918195930316925No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA1738420.18723573814357922No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA1734470.1868103051839566No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAA1648860.17758971893755368No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTCAAAAAAAAA1549740.16691404426469467No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCAAAAAAAAAAAA1528460.16462209151006954No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA1469630.15828583302536114No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTAAAAAAAA1449550.15612312572682394No Hit
CCCTACCTACTATCCAGCGAAACCACAGAAAAAAAAAAAA1447260.15587648231478957No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAAAAAAAAA1433460.15439015956977895No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAA1376410.14824561517826756No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1333580.14363262944139757No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1300000.1400159107618717No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1293090.13927167234389898No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA1293080.13927059529843158No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAA1287590.13867929733682952No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCATAAAAAAAAA1283440.13823232346785894No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA1245270.1341212409187969No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCCAAAAAAAAAA1243440.13392414159826288No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA1240380.13359456568523878No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCAAAAAAAAAAA1205710.1298604490497664No Hit
TCACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAA1147520.12359312147497153No Hit
TCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1126180.12129470644754205No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCAAAAAAAAA1123720.12102975326256189No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAA1115940.12019181188892547No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1098000.11825959232041162No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGCAAAAAAAAAA1096400.11808726504562779No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA1089490.11734302662765508No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAA1070650.11531387296707532No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAA1068270.11505753614583437No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAAA1065060.1147118045507993No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1055810.11371553749345521No Hit
CCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1051100.11320824907831027No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA1050970.11319424748723408No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAA1050520.11314578044120112No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCAAAAAAAAAA1044090.11245324020566355No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA1034430.11141281428415611No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAAAA1033450.11130726382835102No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAA1019260.1097789363101118No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1018530.10970031199099167No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1012070.10900454061905192No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCGTAAAAAAAAA1001630.10788010515108734No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA995590.10722956968877834No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA993660.10702169991357033No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAA955730.10293646645572586No Hit
GCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA949750.10229239326622126No Hit
CCCTACCTACTATCCAGCGAAACCAAAAAAAAAAAAAAAA928740.10002952073921595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)