FastQCFastQC Report
Thu 29 Jun 2023
SRR649756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99189999
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA70438297.1013500060626065No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA60379886.087295151600919No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA42783944.313332032597359No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA21249652.142317795567273No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA14258431.4374866562908222No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA12755931.2860096913601138No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA10997051.1086853625232924No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA10751541.0839338752286911No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA10723071.0810636261827162No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA10707301.0794737481547914No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA10449591.0534922981499375No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA9292460.9368343677470952No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA7541020.7602601145302965No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA7358210.7418298290334694No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA6894700.6951003195392713No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA6676780.6731303626689219No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA5626550.5672497284731296No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA5389860.5433874437280719No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA5176300.5218570473017143No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA4711050.47495211689638184No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA4690480.47287831911360334No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA4356640.4392217001635417No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA4182830.42169876420706487No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA4145530.41793830444539065No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAA4122310.41559734263128684No Hit
TTCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAA4029580.40624861786721056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA3934880.39670128437041313No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAAA3903950.39358302645007587No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA3691280.3721423568115975No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAA3587360.361665494119019No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAA3496160.35247101877680226No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAA3318140.3345236448686727No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA3306740.3333743354508956No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA3256870.3283466108311988No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA2883420.2906966457374397No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAA2760900.2783445939948038No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAA2732310.27546224695495763No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA2728450.27507309481876296No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAA2727230.27495009854773766No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA2705590.27276842698627307No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAA2652850.2674513586798202No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAAA2609870.2631182605415693No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA2608080.2629377987996552No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA2463430.24835467535391345No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAA2397710.24172900737704414No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA2322710.23416776120745803No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAA2270700.22892428903038906No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGATAAAAAAAA2089810.2106875714355033No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA2056390.20731828014233572No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA2028990.2045559048750469No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA2012580.20290150421314151No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA1997620.20139328764384803No Hit
TCACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAA1954700.1970662385025329No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAA1953130.19690795641604955No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA1799850.18145478557772746No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA1797100.18117753988484261No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAA1772500.17869745114121838No Hit
GCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1771510.17859764269177986No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA1730900.17450347993248794No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1714420.17284202210749092No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAA1700580.17144672014766327No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1666480.16800887355589145No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA1650500.16639782403869166No Hit
CCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1643700.16571227105264916No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTAAAAAAAA1608070.1621201750390178No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGATAAAAAAAAA1554270.1566962411200347No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA1548520.15611654558036644No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA1534750.1547283007836304No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA1518150.15305474496476204No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAAAAAAAA1509940.15222704055073133No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1509550.15218772207064948No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA1499230.15114729459771442No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATAAAAAAAAAA1465110.1477074316736307No Hit
CCCTACCTACTATCCAGCGAAACCACAGAAAAAAAAAAAA1461280.14732130403590388No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCAAAAAAAAA1452670.14645327297563537No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA1445810.14576167099265722No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1444240.1456033889061739No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA1440500.14522633476385052No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAA1431060.14427462591263862No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAA1420270.14318681463037417No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1417530.1429105771036453No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA1393570.14049501099400152No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAA1382160.13934469341006847No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAA1337400.1348321416960595No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGATAAAAAAAAAA1329720.13405787008829387No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGTAAAAAAAAAAA1289940.1300473851199454No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAA1278340.12887791237904941No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA1272910.1283304781563714No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA1256410.12666700399906244No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAA1232290.12423530723092355No Hit
ACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA1206780.12166347536710834No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA1163640.1173142465703624No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAA1152610.11620223930035527No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAA1136930.11462143476783379No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAA1130760.11399939624961584No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAA1090960.10998689494895549No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAA1059340.10679907356385798No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAAAA1028570.10369694630201579No Hit
TCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1027630.10360217868335697No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAA1016370.10246698359176311No Hit
CCCTACCTACTATCCAGCGAAACCACAGCAAAAAAAAAAA1013190.10214638675417266No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAA1013090.10213630509261322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)