FastQCFastQC Report
Tue 27 Jun 2023
SRR649757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86455287
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA18678402.1604693764997855No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA14964851.7309352058480818No Hit
CCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAA13290431.537260526357399No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA10988241.2709737462325468No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA10850181.255004798029298No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA10390781.2018675040660036No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA9556321.1053482478173948No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAA8723051.0089666349728272No Hit
ACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAA8082070.9348265768870792No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAA7713310.8921733149760986No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA7599450.8790035015440987No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA7510860.8687565862802584No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAA6196920.7167774482085751No Hit
TTCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAA5770970.6675092062328126No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA5588470.6464000287223614No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAAAAAAAAAAAAA5393290.6238241971251567No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAA5338480.6174845038684563No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAAA4893920.5660637041202582No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAA4706390.5443727229776011No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA4558700.5272899042021572No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAAA4349570.5031005217760713No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGAAAAAAAAAA3841060.444282834894759No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA3836470.4437519246220304No Hit
CCCTACCTACTATCCAGCGAAACCACAGAAAAAAAAAAAA3822620.44214994046575773No Hit
TCACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAA3472520.40165501966351697No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAAAAAAAAAA3409700.39438883593087837No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA3159870.36549181775314676No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAA3101350.3587230009426723No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA2949310.34113703190875994No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCAAAAAAAAAAA2941040.3401804680840398No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA2907050.33624895606442207No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAA2816070.3257255973252393No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAA2779440.32148872514875815No Hit
GGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAAA2696660.31191383356346963No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAA2673050.3091829421606107No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAA2664840.30823331833945566No Hit
CCCTACCTACTATCCAGCGAAACCAAAAAAAAAAAAAAAA2608030.3016622916305859No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA2600690.30081329786112443No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA2386550.2760444251373545No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAA2332780.2698250252757821No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAA2237990.2588609763102169No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAA2166340.2505734553862507No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA2027350.23449693712774325No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1999270.23124901545928592No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAA1916040.22162207384725935No Hit
GCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1911890.22114205693400799No Hit
ACATTGATCATCGACACTTCGAACACAAAAAAAAAAAAAA1896080.21931336599460946No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA1874720.21684272472544103No Hit
CCGACGTACGCAGTTTTATCCGGCAAAAAAAAAAAAAAAA1808470.20917980412233206No Hit
CCCGACGTACGCAGTTTTATCCGGCAAAAAAAAAAAAAAA1799400.20813070691674415No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA1790400.20708970638198218No Hit
CCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAAAA1784050.20635522267134454No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1780650.20596195580265667No Hit
ACATTGATCATCGACACTTCGAACGTAAAAAAAAAAAAAA1764550.2040997215126936No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAA1762300.20383947137900313No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA1729050.199993552736688No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAAA1691280.1956248204924703No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAA1666040.19270539232609338No Hit
TGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA1658770.19186449522745785No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA1637010.18934758726785558No Hit
TTCCCTACCTACTATCCAGCGAAACCAAAAAAAAAAAAAA1616610.18698798605572844No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1584210.18324038413058533No Hit
CACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAA1571870.18181305673070056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAA1524290.17630963390359225No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAA1483020.1715360681180782No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA1480930.17129432466056124No Hit
GGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAAAA1388350.16058589915964305No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA1380930.15972765205209485No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAA1360850.15740506419231481No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAAAAAAAAA1351060.15627268694394594No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA1345810.15566543663200147No Hit
TCGACGTACGCAGTTTTATCCGGCAAAAAAAAAAAAAAAA1330840.15393390574251403No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAA1309090.15141815445017262No Hit
ACATTGATCATCGACACTTCGAACGCTAAAAAAAAAAAAA1287440.14891396983043964No Hit
GGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAAAAA1273570.14730967233964534No Hit
GACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAAA1272230.1471546789266919No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAA1249970.14457993760404728No Hit
TCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1238100.14320697356542234No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA1236470.14301843680190432No Hit
GGGGGGGGGATGCGTGCATTTATCAGTAAAAAAAAAAAAA1221000.14122907254937456No Hit
CCCTACCTACTATCCAGCGAAACCATAAAAAAAAAAAAAA1212020.14019038534913428No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAA1201840.1390128980775924No Hit
CGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAAA1197760.13854097783516697No Hit
TCCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAA1180980.13660009017146632No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1169820.13530924950836146No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAAA1140930.13196763779177553No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA1136320.13143441418452523No Hit
TTGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA1127290.13038994364798073No Hit
TTGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA1121480.12971791996942883No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTCAAAAAAAAA1104750.1277828156420324No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAA1103000.12758039887138423No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAA1090400.12612299812271746No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCCAAAAAAAAAA1074680.12430471718866655No Hit
GGGGGGGGGATGCGTGCATTTATCATAAAAAAAAAAAAAA1073790.12420177380245119No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAA1046520.12104754218212241No Hit
GGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAA1038880.12016384839483558No Hit
GGGGGGGGGATGCGTGCATTTATCACAAAAAAAAAAAAAA1034510.1196583848018456No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAA1031430.11930213128550483No Hit
TCACATTGATCATCGACACTTCGAACGTAAAAAAAAAAAA1030530.11919803123202864No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA1027730.11887416439899158No Hit
TCCCTACCTACTATCCAGCGAAACCAAAAAAAAAAAAAAA1026120.11868794096999528No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA1025920.11866480762477835No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA1024590.11851097087908573No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGAAAAAAAAAAAA1010050.11682917668181472No Hit
TTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAAAA996130.1152190958547162No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTAAAAAAAAAAAAA991260.1146557988986839No Hit
TCACATTGATCATCGACACTTCGAACACAAAAAAAAAAAA980850.11345170828014255No Hit
GACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAAAA974790.11275076792006948No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA942430.10900779266396976No Hit
CATTGATCATCGACACTTCGAACGCAAAAAAAAAAAAAAA937510.10843871237163322No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA936650.10833923898720041No Hit
CCCGACGTACGCAGTTTTATCCGATAAAAAAAAAAAAAAA933400.10796332212742524No Hit
CCCTACCTACTATCCAGCGAAACCACAGCAAAAAAAAAAA930790.10766143197234426No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGAAAAAAAAAAAA926980.10722074174596169No Hit
GACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAAAA922790.10673609816366696No Hit
TCGACTGCATAATTTGTGGTAGTGGGCAAAAAAAAAAAAA909980.10525440740252243No Hit
TTCCCTACCTACTATCCAGCAAAAAAAAAAAAAAAAAAAA904080.1045719737186229No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTAAAAAAAAAAAA897340.10379237998481226No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAA896290.10367092992242337No Hit
ACACATTGATCATCGACACTTCGAACGCAAAAAAAAAAAA891360.10310069296282597No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAA885910.10247030930566456No Hit
CCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA881940.10201111240310844No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAA879570.10173698226228779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)