FastQCFastQC Report
Tue 27 Jun 2023
SRR649758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR649758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97287627
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA74863607.695079251958731No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA73307457.53512571542114No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA41262684.241308095632757No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA20892082.147454989317398No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAA14032451.4423673834700481No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA13030501.339378953091332No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA12931081.3291597707486482No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAA12486051.283416029871918No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA10612731.0908612253436913No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA10459121.075071961617483No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA10335281.0623426964664273No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA8756450.9000579282296606No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAA8420200.8654954653174961No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA6956260.7150200096873572No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA6496420.6677539786225848No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA5804960.5966801924359816No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAA5706100.5865185713698208No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA4835820.49706423613354245No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA4826970.49615456238849365No Hit
CCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAAA4751510.4883981803770381No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA4636170.4765426131732044No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA4338480.44594365530161406No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAA4211810.4329235001281304No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA4154420.427024497164475No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA4009540.41213257262406044No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAA3980090.4091054662069206No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAA3584530.3684466473830223No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA3343390.34366035056030303No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAA3243700.33341341545929576No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAAA3079050.3164893722816366No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAA3041330.3126122091558467No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAA2986420.30696812041679256No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAA2856010.29356353814653124No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAA2667370.27417361099783016No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAA2629410.27027177875353053No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAA2545150.26161086239671566No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAA2478450.2547549032108677No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAA2386590.2453127981012426No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAA2329460.23944052001597282No Hit
GCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA2281440.23450464055413747No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAA2099180.21577050080582189No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAAAAAAA2086940.21451237576182222No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAA2030250.2086853243938204No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAAAAAAAA1908680.19618938798866992No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTAAAAAAAAAA1890330.1943032283026083No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAAAAAAA1879670.19320750828879812No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAA1846480.18979597477488067No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAA1838290.18895414110573383No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAA1819600.18703303350178332No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAA1761700.18108160866129464No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAA1753520.1802408028720857No Hit
TTCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAA1720420.1768385202776094No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAA1648150.16941003196634655No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAA1642870.16886731135913102No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTAAAAAAAAAAAA1589920.16342468708790686No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAAA1584950.16291383075876648No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCAAAAAAAAAAAA1578310.1622313184799954No Hit
TTCCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAA1517280.1559581672189414No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAA1482220.1523544201566351No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAA1460240.1500951400531128No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAA1452310.1492800312623516No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGTTAAAAAAAAAA1433800.14737742549728342No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAA1426870.14666510470031302No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAA1417980.1457513194355126No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAA1405470.1444654416331894No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTAAAAAAAAA1384540.14231408892314745No Hit
TCCCTACCTACTATCCAGCGAAACCACAAAAAAAAAAAAA1339920.13772768864020088No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAA1339290.13766293220411266No Hit
TGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAA1323380.13602757522290065No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAA1303870.13402218146404166No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAA1284200.13200034162617616No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAA1279430.13151004289579393No Hit
TCGACGTACGCAGTTTTATCCGGTAAAAAAAAAAAAAAAA1261140.12963005048935977No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAA1241490.12761026641137008No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTAAAAAAAAAAA1222760.12568504728766794No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAAAAAAA1217220.12511560180206677No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAA1207370.12410314006322716No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGATAAAAAAAA1206270.12399007327005725No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAA1181720.12146662802249251No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAA1163790.11962363929382304No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAAAAA1163580.11960205381512698No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGATAAAAAAAAA1157440.11897093553325132No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAA1145620.11775598144664377No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCTAAAAAAAAA1129750.11612473598518339No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTAAAAAAAAAAA1114550.11456235847956286No Hit
CCCCGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAA1113760.11448115596446812No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCCAAAAAAAAAA1106800.11376575152768399No Hit
CCCTACCTACTATCCAGCGAAACCACAGCAAAAAAAAAAA1098330.11289513722027571No Hit
CCGACGTACGCAGTTTTATCCGGTAAAACAAAAAAAAAAA1071270.11011369410829602No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTAAAAAAAAAA1055250.10846703044776701No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAA1046590.10757688642153848No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAA1030780.10595180823970556No Hit
TCCCTACCTACTATCCAGCGAAACCACAGCCAAAAAAAAA1029320.10580173776877096No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAA997460.1025269122865953No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAAAAAAAA974010.10011653383220048No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAA973610.10007541863468414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)