FastQCFastQC Report
Wed 9 Aug 2023
SRR6507966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93547
Total Bases28 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT3133933.50080708093258No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA2705428.920221920531926No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT10661.139534137919976No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT9981.0668434049194522No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA8580.9171860134477856No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA5910.6317679882839642No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC5700.6093193795632142TruSeq Adapter, Index 3 (97% over 37bp)
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT5560.5943536404160475No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT5390.5761809571659166No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC4550.48638652228291657No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA4300.45966198809154757No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA4280.45752402535623804No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA4190.4479031930473452No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT3450.3687985718408928No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA3390.36238468363496423No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT3360.35917773953199994No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT3260.34848792585545235No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT3210.34314301901717853No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT3090.33031524260532136No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT2950.3153495034581547No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT2710.2896939506344405No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2680.2864870065314762No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT2640.2822110810608571No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCTT2560.273659230119619No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT2520.269383304649No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA2420.25869349097245237No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGTCAGCGTTGAACCAT2410.2576245096047976No Hit
GAACGGTTTGACGTACAGACCATTAAAGGAGTGTAGTAAGGCAAGTCCCT2360.2522796027665238No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCGTGTCAGCGTTGAACCAT2190.23410691951639284No Hit
AGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAT2160.23089997541342855No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT2070.2212791431045357No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGACAGCGTTGAACCA2020.2159342362662619No Hit
GAACGGTTTGACGTACAGACCATTAAAGTAGTGTAGTAAGGCAAGTCCCT2020.2159342362662619No Hit
GAGCCATCGTCAGGAGTGATGAGGCCGAAGCCTTTGTCAGCGTTGAACCA1890.20203747848674997No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG1880.20096849711909523No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTGTCAGCGTTGAACCATT1870.19989951575144047No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA1760.18814072070723808No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT1710.18279581386896426No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTGGAACCA1650.1763819256630357No Hit
GAACGGTTTGACGTACAGACCATTAAAGAAGTGTAGTAAGGCAAGTCCCT1600.17103701882476188No Hit
AACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTT1600.17103701882476188No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT1580.16889905608945235No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT1550.1656921119864881No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT1550.1656921119864881No Hit
GCGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA1520.1624851678835238No Hit
GCACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT1490.1592782237805595No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCACGTCCCT1410.15072637283932142No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1380.14751942873635712No Hit
GAACGGTTTGACGTACAGACCATTACAGCAGTGTAGTAAGGCAAGTCCCT1350.14431248463339283No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCACCGTTGAACCA1340.1432435032657381No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGGTGAACCA1320.14110554053042856No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGCTGAACCA1310.1400365591627738No Hit
GAGCCCTCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA1310.1400365591627738No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT1300.13896757779511903No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTACGGCAAGTCCCT1260.1346916523245No Hit
GAACGGTTTGACGTACAGACCATTAAGGCAGTGTAGTAAGGCAAGTCCCT1250.1336226709568452No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCCTGTCAGCGTTGAACCAT1200.1282777641185714No Hit
GCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCATT1200.1282777641185714No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT1140.12186387591264283No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCCTTGTCAGCGTTGAACCA1120.11972591317733332No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAACCTTTGTCAGCGTTGAACCA1110.11865693180967857No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAGGGCAAGTCCCT1110.11865693180967857No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTATCAGCGTTGAACCA1070.11438100633905951No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT1050.11224304360375No Hit
GAGCCATCGTCAGGAGTGATGAAGCTGAAGCCTTTGTCAGCGTTGAACCA1040.11117406223609522No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTCTGTCAGCGTTGAACCA1030.11010508086844047No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT1030.11010508086844047No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTTTCAGCGTTGAACCA1020.1090360995007857No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTTTACGATACCAGTCATTTTACC970.10369119266251188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)