FastQCFastQC Report
Wed 9 Aug 2023
SRR6507967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114735
Total Bases34.4 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT2028617.680742580729508No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA104379.096613936462283No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG52244.553100623175143No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT17121.4921340480237069TruSeq Adapter, Index 14 (97% over 44bp)
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT12501.0894670327275895No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT12141.0580903821850351No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT11931.0397873360352117No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT11400.9935939338475617No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT9440.8227655031158756No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG9000.7844162635638646No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA8120.7077177844598422No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT7940.692029459188565No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT7430.6475792042532793No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA7240.63101930535582No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA6940.6048720965703578No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG6850.5970279339347191No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT6460.5630365625136183No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT6200.5403756482328844No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT6100.5316599119710638No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT6070.5290451910925176No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC5820.5072558504379657No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT5690.4959253932975988No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT5500.4793654944001395No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCATTGAACCA5280.4601908746241339No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT5010.4366583867172179No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT4910.42794265045539726No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT4610.4017954416699351No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG4270.37216193837974465No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA4250.3704187911273805No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT4240.3695472175011984No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT4080.3556020394822853No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA4050.35298731860373905No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCCTTGAACCA3950.34427158234191835No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA3930.3425284350895542No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA3880.33817056695864384No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT3750.3268401098182769No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAAGGAATGTGCACTACGAGGG3730.32509696256591275No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG3580.31202335817318166No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT3380.29459188564954025No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA3260.2841330021353554No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTATCAGCGTTGAACCA3250.2832614285091733No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTCCACTACGAGGG3230.28151828125680917No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT3120.2719309713688064No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT3020.2632152351069857No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT2930.255371072471347No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA2770.24142589445243387No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTTTCAGCGTTGAACCA2620.2283522900597028No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT2450.2135355384146076No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2400.20917767028369721No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCTTTGAACCA2350.20481980215278686No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCGTTAAAGG2220.19348934501241993No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTTTACGATACCAGTCATTTTACC2070.18041574061968885No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT1950.16995685710550398No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1930.16821370985313985No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA1930.16821370985313985No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT1860.16211269446986534No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA1790.15601167908659083No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGCTGAACCA1760.1533969582080446No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGACAGCGTTGAACCA1740.15165381095568048No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTGCCT1580.13770863293676733No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGATGAACCA1570.13683705931058526No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTGGAACCA1560.1359654856844032No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTTGTAAGGCAAGTCCCT1530.13335076480585698No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTAGAACCA1490.1298644703011287No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC1440.12550660217021833No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGGAAGTCCCT1430.12463502854403626No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGGAAGTCCCT1330.11591929228221554No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGTCAGCGTTGAACCAT1310.1141761450298514No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT1300.11330457140366933No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA1270.1106898505251231No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCACCGTTGAACCA1260.10981827689894104No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT1180.10284568788948445No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAACCTTTGTCAGCGTTGAACCA1180.10284568788948445No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCACGTCCCT1150.10023096701093825No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACAAGGG1150.10023096701093825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)