FastQCFastQC Report
Wed 9 Aug 2023
SRR6507968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109450
Total Bases32.8 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT1412812.908177249885794No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG68206.231155778894473No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA55985.11466423024212No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG14961.3668341708542713No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT13801.2608497030607584No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT12701.1603471904979443No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC11481.0488807674737322No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG10440.9538602101416173No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT9700.8862494289629967No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT9700.8862494289629967No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAAGGAATGTGCACTACGAGGG8990.821379625399726No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT8730.797624486066697No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT7630.697121973503883No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT6530.596619460941069No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT6460.5902238465052535No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT6410.5856555504796711No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT6410.5856555504796711No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG6390.5838282320694381No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT6250.5710370031978073No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT5740.5244403837368661No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA5630.5143901324805847No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG5260.4805847418912746No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT4930.4504339881224303No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT4710.4303334856098675No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA4630.4230242119689356No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT4540.41480127912288717No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT4540.41480127912288717No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA4510.4120603015075377No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA4470.40840566468707173No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA4260.38921882137962543No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT4220.3855641845591595No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT4160.3800822293284605No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT4150.379168570123344No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTCCACTACGAGGG3860.35267245317496576No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT3720.33988122430333484No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCGTTAAAGG3560.325262677021471No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT3480.31795340338053907No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA3450.3152124257651896No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCATTGAACCA3220.29419826404751026No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT2530.23115577889447234No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA2440.22293284604842392No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA2400.21927820922795796No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTTGTAAGGCAAGTCCCT2370.2165372316126085No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA2340.21379625399725902No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT2340.21379625399725902No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCCTTGAACCA2340.21379625399725902No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA2180.19917770671539514No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT2170.19826404751027865No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2010.1836455002284148TruSeq Adapter, Index 18 (97% over 40bp)
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGAATGTGCA1810.16537231612608497No Hit
GAGCCATCGTCAGGAGTGATGAAGCGATAAAGGAATGTGCACTACGAGGG1790.163544997715852No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTTTCAGCGTTGAACCA1770.161717679305619No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCTTTGAACCA1740.15897670169026953No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT1720.15714938328003655No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA1660.15166742804933758No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAGGAATGTGCACTACGAGGGG1630.1489264504339881No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA1610.14709913202375513No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTATCAGCGTTGAACCA1570.14344449520328917No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCACGTCCCT1560.14253083599817268No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT1510.13796253997259023No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGGAAGTCCCT1480.13522156235724073No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGGAAGTCCCT1480.13522156235724073No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTGCCT1470.13430790315212424No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACAAGGG1400.1279122887163088No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATGTGCACTACGAGGG1360.12425765189584285No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAAGGAAAGTGCACTACGAGGG1360.12425765189584285No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT1310.1196893558702604No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCTCT1250.11420740063956143No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAGGTGCACTACGAGGG1220.11146642302421196No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTGGAACCA1210.11055276381909547No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT1200.10963910461397898No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTCCCTTCAA1190.10872544540886249No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGCTGAACCA1190.10872544540886249No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTTTACGATACCAGTCATTTTACC1190.10872544540886249No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGAGCACTACGAGGG1180.107811786203746No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCTCT1170.10689812699862951No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA1150.10507080858839653No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1140.10415714938328004No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGACTTGCC1120.10232983097304706No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGACAGCGTTGAACCA1100.10050251256281408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)