FastQCFastQC Report
Wed 9 Aug 2023
SRR6507969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131552
Total Bases39.4 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT1848214.049197275602044No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA91226.934140111894917No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG50743.85702991972756No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT17761.3500364874726345No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC15651.1896436390172709No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT14451.0984249574312819No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG13541.029250790561907No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT12520.9517149112138166No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATGTGCACTACGAGGG10430.7928423741182195No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG10270.7806798832400876No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA9420.7160666504500122No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT7870.59824252006811No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA7280.5533933349549988No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT5870.44621138409146194No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATATGCACTACGAGGG5800.4408902943322792No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT5610.42644733641449767No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT5570.42340671369496474No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT5500.418085623935782No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT5440.4135246898564826No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT5250.39908173193870106No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTAAAGGAATGTGCACTACGAGGG5150.3914801751398686No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT5100.38767939674045243No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT5060.3846387740209195No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA5050.3838786183410362No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT5020.3815981513013865No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA4880.3709559717830212No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT4770.3625942593043055No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT4560.34663099002675746No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATTTGCACTACGAGGG4460.33902943322792506No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCTT4430.3367489661882753No Hit
GAGCCATCGTCAGGAGTGATGAAGCTTTAAAGGAATGTGCACTACGAGGG4280.32534663099002675No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT4250.323066163950377No Hit
GAACGGTTTGACGTACAGACCATTAAAGGAGTGTAGTAAGGCAAGTCCCT4180.31774507419119435No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA4070.3093833617124787No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT3650.2774568231573826No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT3560.2706154220384335No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAAGGAATGTGCACTACGAGGG3530.26833495499878374No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA3500.266054487959134No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT3400.25845293116030166No Hit
GAACGGTTTGACGTACAGACCATTAAAGTAGTGTAGTAAGGCAAGTCCCT3400.25845293116030166No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT3340.2538919970810022No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT3060.23260763804427145No Hit
GAACGGTTTGACGTACAGACCATTACAGCAGTGTAGTAAGGCAAGTCCCT3040.23108732668450496No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGACTTGCC3020.2295670153247385No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT3000.228046703964972No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT2870.21816468012648993TruSeq Adapter, Index 19 (97% over 40bp)
GAACGGTTTGACGTACAGACCATTATAGCAGTGTTGTAAGGCAAGTCCCT2870.21816468012648993No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT2780.21132327900754075No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA2760.20980296764777426No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGAGCACTACGAGGG2730.20752250060812452No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG2630.19992094380929215No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2560.19459985405010946No Hit
GAACGGTTTGACGTACAGACCATTAAAGAAGTGTAGTAAGGCAAGTCCCT2530.19231938701045975No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG2470.1877584529311603No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA2400.18243736317197762No Hit
GAGCCATCGTCAGGAGTGATGAAGGAAAGTGCACTACGAGGGGTATCAAC2390.18167720749209437No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT2340.17787642909267817No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT2270.1725553393334955No Hit
GAGCCATCGTCAGGAGTGATGAAGCGATAAAGGAATGTGCACTACGAGGG2260.17179518365361227No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTCCACTACGAGGG2260.17179518365361227No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT2170.1649537825346631No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA2080.15811238141571393No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA1990.15127098029676478No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAAAGTGCACTACGAGGG1970.1497506689369983No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATATGCACTACGAGGGGTATCAAC1830.13910848941863294No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA1830.13910848941863294No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA1780.13530771101921674No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATATGCACTACGAGGG1740.13226708829968378No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT1700.12922646558015083No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGTATCAAC1700.12922646558015083No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTACCAAC1700.12922646558015083No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTACCAACGATAACTCTTGAAGGG1600.12162490878131842No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC1560.11858428606178546No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCACGTCCCT1550.1178241303819022No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTATAA1540.11706397470201896No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1530.11630381902213573No Hit
GAACGGTTTGACGTACAGACCATTAGAGCAGTGTAGTAAGGCAAGTCCCT1500.114023351982486No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATTTGCACTACGAGGGGTATCAAC1360.10338117246412065No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTTGTAAGGCAAGTCCCT1350.10262101678423742No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGAG1340.10186086110435418No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTACGAGGGGTATCAACGATAACT1330.10110070542447094No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTGCCT1320.1003405497445877No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)