FastQCFastQC Report
Wed 9 Aug 2023
SRR6507970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118571
Total Bases35.5 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT2103917.743799073972557No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA1211110.214133304096281No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG38983.287481761982272No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT23792.0063927941908224No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT12821.08120872726046No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT12151.024702498924695No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATGTGCACTACGAGGG10800.9108466657108399No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC10750.9066297829992156No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG8110.6839783758254548No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA7010.5912069561697211No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCTT6180.5212067031567584No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATATGCACTACGAGGG6170.5203633266144336No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTAAAGGAATGTGCACTACGAGGG5510.4647004748209933No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG5440.45879683902471935No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA5320.44867632051682116No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA5020.42337502424707557No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT4940.41662801190847676No Hit
GAACGGTTTGACGTACAGACCATTAAAGGAGTGTAGTAAGGCAAGTCCCT4680.3947002218080306No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT4650.39217009218105603No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA4580.38626645638478213No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGACTTGCC4580.38626645638478213No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATTTGCACTACGAGGG4410.37192905516525965No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT4400.3710856786229348No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA4360.3677121724536354No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT4250.35843503048806197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4060.3424108761838898TruSeq Adapter, Index 15 (97% over 40bp)
GAGCCATCGTCAGGAGTGATGAAGCTTTAAAGGAATGTGCACTACGAGGG4010.3381939934722656No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT3960.3339771107606413No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA3910.32976022804901706No Hit
GAACGGTTTGACGTACAGACCATTAAAGTAGTGTAGTAAGGCAAGTCCCT3910.32976022804901706No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT3770.31795295645646915No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT3760.3171095799141443No Hit
GAACGGTTTGACGTACAGACCATTACAGCAGTGTAGTAAGGCAAGTCCCT3740.3154228268294946No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTTGTAAGGCAAGTCCCT3440.290121530559749No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT3400.2867480243904496No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT3340.2816877651365005No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT3160.26650698737465317No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT3040.256386468866755No Hit
GAACGGTTTGACGTACAGACCATTAAAGAAGTGTAGTAAGGCAAGTCCCT2860.2412056911049076No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT2820.2378321849356082No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGAGCACTACGAGGG2730.23024179605468453No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA2710.22855504297003482No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT2600.21927790100446146No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT2600.21927790100446146No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA2560.21590439483516205No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC2450.20662725286958872No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT2420.20409712324261411No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGTATCAAC2410.20325374670028926No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT2350.19819348744634016No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT2300.1939766047347159No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT2250.18975972202309163No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT2220.18722959239611708No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2190.18469946276914254No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA2120.17879582697286858No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCTT2080.17542232080356915No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA2080.17542232080356915No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAAAGTGCACTACGAGGG2070.17457894426124432No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT2050.17289219117659463No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATATGCACTACGAGGG2020.17036206154962005No Hit
GAACGGTTTGACGTACAGACCATTAGAGCAGTGTAGTAAGGCAAGTCCCT1990.1678319319226455No Hit
GAGCCATCGTCAGGAGTGATGAAGCGATAAAGGAATGTGCACTACGAGGG1800.15180777761847333No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATATGCACTACGAGGGGTATCAAC1770.14927764799149876No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAACCTTTGTCAGCGTTGAACCA1710.14421738873754966No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT1700.1433740121952248No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGTCAGCGTTGAACCAT1620.136626999856626No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT1610.13578362331430113No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA1580.13325349368732659No Hit
GAGCCATCGTCAGGAGTGATGAAGGAAAGTGCACTACGAGGGGTATCAAC1580.13325349368732659No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT1540.12987998751802718No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT1540.12987998751802718No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG1460.12313297517942837No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1430.1206028455524538No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATCTGCACTACGAGGG1420.11975946901012895No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGAG1400.11807271592547924No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTAAAAGGAATGTGCACTACGAGGG1350.11385583321385499No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATTTGCACTACGAGGG1340.11301245667153013No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATTTGCACTACGAGGGGTATCAAC1320.11132570358688043No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTTTCAGCGTTGAACCA1260.10626544433293132No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG1230.10373531470595676No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTCTGTCAGCGTTGAACCA1210.10204856162130706No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTAAAGGAAAGTGCACTACGAGGG1210.10204856162130706No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT1190.10036180853665735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)