FastQCFastQC Report
Tue 8 Aug 2023
SRR6507971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136697
Total Bases41 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT2512218.37787222835907No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA133969.799776147245367No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG51753.7857451151085977No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT24141.7659495087675663No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT14411.0541562726321718No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT13891.016115935243641No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT12330.9019949230780486No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA11480.8398136023467963No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATGTGCACTACGAGGG11250.8229880685018691No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC10390.7600752028208374No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG8360.6115715780156111No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATATGCACTACGAGGG7080.517933824443843No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA6960.5091552850464897No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCTT6880.5033029254482542No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA6780.4959874759504598No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG6340.46379949816016447No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA6190.4528263239134729No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT5730.4191752562236186No Hit
GAACGGTTTGACGTACAGACCATTAAAGGAGTGTAGTAAGGCAAGTCCCT5650.41332289662538313No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT5580.4082020819769271No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT5320.38918191328266166No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATTTGCACTACGAGGG5140.3760141041866317No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTAAAGGAATGTGCACTACGAGGG4920.3599201152914841No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT4820.3526046657936897No Hit
GAACGGTTTGACGTACAGACCATTAAAGTAGTGTAGTAAGGCAAGTCCCT4720.3452892162958953No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA4520.3306583173003065No Hit
GAACGGTTTGACGTACAGACCATTACAGCAGTGTAGTAAGGCAAGTCCCT4290.31383278345537946No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT4190.306517333957585No Hit
GAGCCATCGTCAGGAGTGATGAAGCTTTAAAGGAATGTGCACTACGAGGG4110.3006649743593495No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT4090.2992018844597906No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT4070.2977387945602318No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCGTTAAAGG4040.29554415971089343No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA3980.29115489001221684No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT3500.2560407324228037No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT3460.25311455262368593No Hit
GAACGGTTTGACGTACAGACCATTAAAGAAGTGTAGTAAGGCAAGTCCCT3340.2443360132263327No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT3190.2333628389796411No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGAGCACTACGAGGG3080.22531584453206727No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTTGTAAGGCAAGTCCCT2970.21726885008449345No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA2880.21068494553647849No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT2870.20995340058669906No Hit
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT2870.20995340058669906No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2820.20629567583780187No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT2800.204832585938243No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC2770.20263795108890464TruSeq Adapter, Index 16 (98% over 50bp)
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA2680.1960540465408897No Hit
GAACGGTTTGACGTACAGACCATTTAATTATTAAAGGTAATACACTATGT2620.19166477684221306No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT2610.19093323189243364No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA2560.18727550714353644No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA2550.186543962193757No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT2480.18142314754530092No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT2400.1755707879470654No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT2310.16898688339905044No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAACCTTTGTCAGCGTTGAACCA2270.1660607035999327No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT2240.16386606875059437No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC2210.16167143390125607No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA2200.16093988895147662No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA2190.1602083440016972No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA2140.1565506192528No Hit
GAACGGTTTGACGTACAGACCATTAGAGCAGTGTAGTAAGGCAAGTCCCT2120.1550875293532411No Hit
GAACGGTTTGACGTACAGACCATTAATTATTAAAGGTAATACACTATGTC2090.15289289450390278No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGTCAGCGTTGAACCAT2060.15069825965456446No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT2000.14630898995588784No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT1900.13899354045809345No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGACTTGCC1830.13387272580963738No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGAATGTGCA1770.12948345611096074No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTTTCAGCGTTGAACCA1770.12948345611096074No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT1750.12802036621140184No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT1740.12728882126162241No Hit
GAGCCATCGTCAGGAGTGATGAAGCGATAAAGGAATGTGCACTACGAGGG1690.12363109651272522No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTATCAGCGTTGAACCA1690.12363109651272522No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCTT1660.12143646166338691No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCACCGTTGAACCA1650.12070491671360747No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT1560.1141210121655925No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGAG1540.11265792226603363No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCATTGAACCA1540.11265792226603363No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCCTGTCAGCGTTGAACCAT1530.1119263773162542No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATCTGCACTACGAGGG1520.11119483236647476No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG1450.10607401771801869No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAAGG1410.10314783791890093No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTCTGTCAGCGTTGAACCA1410.10314783791890093No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGTG1400.1024162929691215No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG1380.1009532030695626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)