FastQCFastQC Report
Wed 9 Aug 2023
SRR6507972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6507972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118072
Total Bases35.4 Mbp
Sequences flagged as poor quality0
Sequence length300
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCT2109317.864523341689818No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCA117999.993055085032863No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGGG47964.061928314926486No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCCT25322.1444542313164847No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTTGTAAGGCAAGTCCCT13791.1679314316688123No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTAGTAAGGCAAGTCCCT12991.1001761636967275No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATGTGCACTACGAGGG12841.0874720509519615No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTATGTCAGCGTTGAACCA8820.7470018293922353No Hit
GAGCCATCGTCAGGAGTGATGAAGGAATGTGCACTACGAGGGGTATCAAC8100.6860220882173589No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAAAGTGCACTACGAGGG7940.6724710346229419No Hit
GAGCCATCGTCAGGAGTGATGAAGGGGTATCAACGATAACTCTTGAAGGG7680.6504505725320144No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCT7230.6123382342977167No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATATGCACTACGAGGG7140.604715766650857No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCTT6810.5767667186123722No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATTTGCACTACGAGGG5850.4954603970458703No Hit
GAACGGTTTGACGTACAGACCATTAAAGGAGTGTAGTAAGGCAAGTCCCT5710.4836032251507555No Hit
GAACGGTTTGACGTACAGACCATTAAGCAGTGTAGTAAGGCAAGTCCCTT5560.4708991124059896No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTAAAGGAATGTGCACTACGAGGG5470.46327664475913No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTAGTCAGCGTTGAACCA5400.4573480588115726No Hit
GAGCCATCGTCAGGAGTGATGAAGTGCACTACGAGGGGTATCAACGATAA5320.4505725320143641No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGGTCAGCGTTGAACCA5250.4446439460668067No Hit
GAGCCATCGTCAGGAGTGATGAAGCTTTAAAGGAATGTGCACTACGAGGG5170.43786841926959824No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTTTAA4830.4090724303814621No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTTAGGCAAGTCCCT4810.40737854868216006No Hit
GAACGGTTTGACGTACAGACCATTAAAGTAGTGTAGTAAGGCAAGTCCCT4580.38789890914018565No Hit
GAACGGTTTGACGTACAGACCATTACAGCAGTGTAGTAAGGCAAGTCCCT4540.3845111457415814No Hit
GAACGGTTTGACGTACAGACCTTTAAAGCAGTGTAGTAAGGCAAGTCCCT4520.3828172640422793No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCTAGTCCCT4350.36841926959821125No Hit
GAACGGTTTGACGTACAGACCATTAATGCAGTGTAGTAAGGCAAGTCCCT4180.3540212751541432No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTTGTAAGGCAAGTCCCT3800.3218375228674029No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTTCCT3770.31929670031844976No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCCAGTCCCT3590.3040517650247307No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATTGTCAGCGTTGAACCA3580.30320482417507966No Hit
GAGCCATCGTCAGGAGTGATGAAGGGTATCAACGATAACTCTTGAAGGGA3510.2972762382275222No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCTCT3440.2913476522799648No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGAGCACTACGAGGG3210.2718680127379904No Hit
GAACGGTTTGACGTACAGACCATTAAAGAAGTGTAGTAAGGCAAGTCCCT3190.27017413103868826No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCATGTCCCT3180.26932719018903717No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTCGTAAGGCAAGTCCCT3090.2617047225421777No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTATGGCAAGTCCCT2920.24730672809810963No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTACCT2870.24307202384985432No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2860.24222508300020326TruSeq Adapter, Index 20 (97% over 44bp)
GAACGGTTTGACGTACAGACCATTAAAGCTGTGTAGTAAGGCAAGTCCCT2620.22189850260857782No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCACT2540.21512297581136935No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAAGCCTTTGTCAGCGTTGAACCA2510.21258215326241614No Hit
GAGCCATCGTCAGGAGTGATGAAGCCAAGCCTTTGTCAGCGTTGAACCAT2500.21173521241276508No Hit
GAACGGTTTGACGTACAGACCATTAAAGCCGTGTAGTAAGGCAAGTCCCT2470.2091943898638119No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCATGTCAGCGTTGAACCAT2460.20834744901416083No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGGAAGTCCCT2360.19987804051765026No Hit
GAACGGTTTGACGTACAGACCATTAGAGCAGTGTAGTAAGGCAAGTCCCT2300.1947963954197439No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGATGAACCA2150.18209228267497798No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGCCAGCGTTGAACCA2060.17446981502811845No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTTTGTCAGCGTTGAACCATTTTAC1970.1668473473812589No Hit
GAACGGTTTGACGTACAGACCATTTAAGCAGTGTTGTAAGGCAAGTCCCT1950.16515346568195677No Hit
GAGCCATCGTCAGGAGTGATGAAGCCCAAGCCTTTGTCAGCGTTGAACCA1880.15922487973439933No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTAGAACCA1820.154143234636493No Hit
GAACGGTTTGACGTACAGACCATTATAGCAGTGTAGTAAGGCAAGTCCTT1780.15075547123788874No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCGCT1780.15075547123788874No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAGGTCCCT1750.14821464868893555No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTGTCAGCGTTGAACCAT1710.14482688529033133No Hit
GAGCCATCGTCAGGAGTGATGAAGCCTAAGCCTTTGTCAGCGTTGAACCA1700.14397994444068027No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATATGCACTACGAGGG1660.14059218104207602No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAAAGTGCACTACGAGGG1650.13974524019242496No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTCTGTCAGCGTTGAACCA1650.13974524019242496No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGTAAGTCCCT1640.1388982993427739No Hit
GAGCCATCGTCAGGAGTGATGAAGCGATAAAGGAATGTGCACTACGAGGG1580.13381665424486755No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATCTGCACTACGAGGG1530.12958194999661224No Hit
GAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGAAAGTCCCT1520.12873500914696118No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGGGTATCAA1500.12704112744765905No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTTCACTACGAGGG1490.126194186598008No Hit
GAACGGTTTGACGTACAGACCTTAAAGCAGTGTAGTAAGGCAAGTCCCTT1480.12534724574835693No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTAAAGGAATTTGCACTACGAGGG1470.12450030489870587No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAACCTTTGTCAGCGTTGAACCA1450.12280642319940376No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTACACTACGAGGG1410.11941865980079952No Hit
GAGCCATCGTCAGGAGTGATGAAGCATTACGAGGGGTATCAACGATAACT1340.11349007385324208No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGTG1310.11094925130428891No Hit
GAGCCATCGTCAGGAGTGATGAAGGGACTTGCCTTACTACACTGCTATAA1300.11010231045463785No Hit
GAGCCATCGTCAGGAGTGATGAAGCGTTAAAGGAATGTGCACTACGAGAG1290.10925536960498679No Hit
GTACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGGAATGTGCA1260.10671454705603362No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCATTGAACCA1210.1024798428077783No Hit
GAGCCATCGTCAGGAGTGATGAAGCCGAAGCCGTGTCAGCGTTGAACCAT1200.10163290195812724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)